Participation of Xenopus Elr-type proteins in vegetal mRNA localization during oogenesis
Directional transport of specific mRNAs is of primary biological relevance. In Xenopus oocytes, mRNA localization to the vegetal pole is important for germ layer formation and germ cell development. Using a biochemical approach, we identified Xenopus Elr-type proteins, homologs of the Hu/ELAV proteins, as novel components of the vegetal mRNA localization machinery. They bind specifically to the localization elements of several different vegetally localizing Xenopus mRNAs, and they are part of one RNP together with other localization proteins, such as Vg1RBP and XStaufen 1. Blocking Elr-type protein binding by either localization element mutagenesis or antisense morpholino oligonucleotide-mediated masking of their target RNA structures, as well as overexpression of wild type and mutant ElrB proteins, interferes with vegetal localization in Xenopus oocytes.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2009 |
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| Erschienen: |
2009 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:284 |
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| Enthalten in: |
The Journal of biological chemistry - 284(2009), 30 vom: 24. Juli, Seite 19982-92 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Arthur, Patrick K [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 28.09.2009 Date Revised 20.10.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1074/jbc.M109.009928 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM188677046 |
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| 100 | 1 | |a Arthur, Patrick K |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Participation of Xenopus Elr-type proteins in vegetal mRNA localization during oogenesis |
| 264 | 1 | |c 2009 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
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| 500 | |a Date Completed 28.09.2009 | ||
| 500 | |a Date Revised 20.10.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Directional transport of specific mRNAs is of primary biological relevance. In Xenopus oocytes, mRNA localization to the vegetal pole is important for germ layer formation and germ cell development. Using a biochemical approach, we identified Xenopus Elr-type proteins, homologs of the Hu/ELAV proteins, as novel components of the vegetal mRNA localization machinery. They bind specifically to the localization elements of several different vegetally localizing Xenopus mRNAs, and they are part of one RNP together with other localization proteins, such as Vg1RBP and XStaufen 1. Blocking Elr-type protein binding by either localization element mutagenesis or antisense morpholino oligonucleotide-mediated masking of their target RNA structures, as well as overexpression of wild type and mutant ElrB proteins, interferes with vegetal localization in Xenopus oocytes | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a ELAV-Like Protein 2 |2 NLM | |
| 650 | 7 | |a ELAVL1 protein, Xenopus |2 NLM | |
| 650 | 7 | |a IGF2BP3 protein, Xenopus |2 NLM | |
| 650 | 7 | |a RNA, Messenger |2 NLM | |
| 650 | 7 | |a RNA-Binding Proteins |2 NLM | |
| 650 | 7 | |a Ribonucleoproteins |2 NLM | |
| 650 | 7 | |a STAU1 protein, Xenopus |2 NLM | |
| 650 | 7 | |a Xenopus Proteins |2 NLM | |
| 650 | 7 | |a elavl2 protein, Xenopus |2 NLM | |
| 700 | 1 | |a Claussen, Maike |e verfasserin |4 aut | |
| 700 | 1 | |a Koch, Susanne |e verfasserin |4 aut | |
| 700 | 1 | |a Tarbashevich, Katsiaryna |e verfasserin |4 aut | |
| 700 | 1 | |a Jahn, Olaf |e verfasserin |4 aut | |
| 700 | 1 | |a Pieler, Tomas |e verfasserin |4 aut | |
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