Infectious RNA transcripts derived from cloned cDNA of a pepino mosaic virus isolate
For the first time, a full-length cDNA clone of the RNA genome of pepino mosaic virus (PepMV) was constructed. RNA was extracted from purified virions of isolate PepMV-Pa and used for cDNA synthesis. The full-length cDNA was produced as one 6.4-kb fragment representing the entire PepMV genome. This fragment was ligated into the pCR-XL-TOPO vector downstream of T7 RNA polymerase promoter, which was included in the 5' primer sequence used for RT-PCR. The PepMV-Pa RNA transcripts obtained were infectious in different host plants, causing symptoms indistinguishable from those of the wild-type isolate. The presence and authenticity of the progeny virus were verified by ELISA, RT-PCR and nucleotide sequencing.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2009 |
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| Erschienen: |
2009 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:154 |
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| Enthalten in: |
Archives of virology - 154(2009), 5 vom: 22., Seite 853-6 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Hasiów-Jaroszewska, Beata [VerfasserIn] |
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| Links: |
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| Themen: |
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| Anmerkungen: |
Date Completed 12.05.2009 Date Revised 13.12.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1007/s00705-009-0368-y |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM187527873 |
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| 245 | 1 | 0 | |a Infectious RNA transcripts derived from cloned cDNA of a pepino mosaic virus isolate |
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| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a For the first time, a full-length cDNA clone of the RNA genome of pepino mosaic virus (PepMV) was constructed. RNA was extracted from purified virions of isolate PepMV-Pa and used for cDNA synthesis. The full-length cDNA was produced as one 6.4-kb fragment representing the entire PepMV genome. This fragment was ligated into the pCR-XL-TOPO vector downstream of T7 RNA polymerase promoter, which was included in the 5' primer sequence used for RT-PCR. The PepMV-Pa RNA transcripts obtained were infectious in different host plants, causing symptoms indistinguishable from those of the wild-type isolate. The presence and authenticity of the progeny virus were verified by ELISA, RT-PCR and nucleotide sequencing | ||
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| 700 | 1 | |a Pospieszny, Henryk |e verfasserin |4 aut | |
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