Loss of REEP4 causes paralysis of the Xenopus embryo
Members of the REEP (Receptor expression enhancing protein) family contain a TB2/DP1, HVA22 domain that is involved in intracellular trafficking and secretion. Consistent with the presence of this domain, REEP1 and REEP3 enhance the expression of odorant and taste receptors in mammals, while mutation of these genes causes defects in neural development. REEP4 was identified in the course of a functional antisense morpholino oligonucleotide screen searching for genes involved in the early development of Xenopus tropicalis: although over-expression of the gene causes no phenotype, embryos lacking REEP4 develop a slightly kinked body axis and are paralysed. At tailbud stages of development, REEP4 is expressed in the somites and neural tube. The paralysis observed in embryos lacking REEP4 might therefore be caused by defects in the nervous system or in muscle. To address this point, we examined the expression of various neural and muscle markers and found that although all are expressed normally at early stages of development, many are down regulated by the tailbud stage. This suggests that REEP4 plays a role in the maintenance of both the nervous system and the musculature.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2009 |
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| Erschienen: |
2009 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:53 |
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| Enthalten in: |
The International journal of developmental biology - 53(2009), 1 vom: 01., Seite 37-43 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Argasinska, Joanna [VerfasserIn] |
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| Links: |
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| Themen: |
Amphibian Proteins |
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| Anmerkungen: |
Date Completed 22.06.2009 Date Revised 19.11.2015 published: Print Citation Status MEDLINE |
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| doi: |
10.1387/ijdb.072542ja |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM185555322 |
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| 100 | 1 | |a Argasinska, Joanna |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Loss of REEP4 causes paralysis of the Xenopus embryo |
| 264 | 1 | |c 2009 | |
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| 500 | |a Date Completed 22.06.2009 | ||
| 500 | |a Date Revised 19.11.2015 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Members of the REEP (Receptor expression enhancing protein) family contain a TB2/DP1, HVA22 domain that is involved in intracellular trafficking and secretion. Consistent with the presence of this domain, REEP1 and REEP3 enhance the expression of odorant and taste receptors in mammals, while mutation of these genes causes defects in neural development. REEP4 was identified in the course of a functional antisense morpholino oligonucleotide screen searching for genes involved in the early development of Xenopus tropicalis: although over-expression of the gene causes no phenotype, embryos lacking REEP4 develop a slightly kinked body axis and are paralysed. At tailbud stages of development, REEP4 is expressed in the somites and neural tube. The paralysis observed in embryos lacking REEP4 might therefore be caused by defects in the nervous system or in muscle. To address this point, we examined the expression of various neural and muscle markers and found that although all are expressed normally at early stages of development, many are down regulated by the tailbud stage. This suggests that REEP4 plays a role in the maintenance of both the nervous system and the musculature | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Amphibian Proteins |2 NLM | |
| 650 | 7 | |a Biomarkers |2 NLM | |
| 650 | 7 | |a Membrane Transport Proteins |2 NLM | |
| 700 | 1 | |a Rana, Amer A |e verfasserin |4 aut | |
| 700 | 1 | |a Gilchrist, Michael J |e verfasserin |4 aut | |
| 700 | 1 | |a Lachani, Kim |e verfasserin |4 aut | |
| 700 | 1 | |a Young, Alice |e verfasserin |4 aut | |
| 700 | 1 | |a Smith, James C |e verfasserin |4 aut | |
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