Regeneration of beta cells in the native pancreata after syngeneic and allogeneic pancreas transplantations in spontaneously type 2 diabetic Torii rats

BACKGROUND: We previously demonstrated that syngeneic pancreas transplantation has a potential to reverse diabetes even in a rat model of type 2 diabetes mellitus, namely Spontaneously Diabetic Torii (SDT; RT1a). The onset of diabetes was significantly delayed in the pancreas transplant recipients. We speculated that perfect diabetic control achieved by pancreas transplantation showed a beneficial effect on the native pancreata & recipients.

MATERIALS AND METHODS: Twenty-five-week-old diabetic SDT rats were divided into 3 groups: untreated controls and syngeneic and allogeneic transplant recipients. We transplanted pancreaticoduodenal grafts from nondiabetic 10-week-old SDT rats and from 10-week-old allogeneic Dark Agouti (DA; RT1a) rats using daily administration of FK506.

RESULTS: Untreated SDT rats showed disappearance of pancreatic and duodenal homeobox-1 (PDX-1) expression in the pancreas and a marked decrease in beta-cell mass. Among syngeneic and allogeneic pancreas transplant recipients, islet-like cell clusters were found in the native pancreata. The beta-cell mass at 40 weeks of age was significantly increased in the native pancreata of recipients compared with age-matched controls. Interestingly, we observed the reexpression of PDX-1 in the nuclei of islet-like cell clusters.

CONCLUSIONS: Our results indicated the benefits of avoiding glucose toxicity by pancreas transplantation which induced PDX-1 expression in the native pancreata of type 2 diabetic recipients, resulting in regeneration of beta cells in the native pancreata.

Medienart:

E-Artikel

Erscheinungsjahr:

2008

Erschienen:

2008

Enthalten in:

Zur Gesamtaufnahme - volume:40

Enthalten in:

Transplantation proceedings - 40(2008), 2 vom: 17. März, Seite 438-40

Sprache:

Englisch

Beteiligte Personen:

Shimada, K [VerfasserIn]
Ito, T [VerfasserIn]
Miao, G [VerfasserIn]
Tanemura, M [VerfasserIn]
Komoda, H [VerfasserIn]
Fumimoto, Y [VerfasserIn]
Kawamoto, K [VerfasserIn]
Nishida, T [VerfasserIn]
Kaneto, H [VerfasserIn]
Sawa, Y [VerfasserIn]

Links:

Volltext

Themen:

Blood Glucose
Homeodomain Proteins
Journal Article
Pancreatic and duodenal homeobox 1 protein
Trans-Activators

Anmerkungen:

Date Completed 24.06.2008

Date Revised 01.06.2011

published: Print

Citation Status MEDLINE

doi:

10.1016/j.transproceed.2008.01.019

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM178562270