Cloning and construction of nucleic acid vaccine of FABP gene cDNA from Echinococcus granulosus
Specific primers were designed according to published nucleotide sequence of FABP (fatty acid binding protein) gene in the GenBank database. The kozak sequence (CCACC) was introduced at the upstream of initiator. The total RNA was extracted from protoscoleces of Echinococcus granulosus (Inner Mongol isolate). The FABP gene cDNA fragment was amplified by RT-PCR and cloned into pMD19-T vector for sequencing and analyzing. The cloned FABP gene cDNA was with 402bp. The ORF encoded 133 amino acids. The amplified cDNA fragment was subcloned into pCDNA3.1 (+) vector. The results showed that the nucleic acid vaccine candidate pcDNA-FABP-NM has been constructed.
Medienart: |
Artikel |
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Erscheinungsjahr: |
2007 |
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Erschienen: |
2007 |
Enthalten in: |
Zur Gesamtaufnahme - volume:25 |
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Enthalten in: |
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases - 25(2007), 4 vom: 19. Aug., Seite 352-4 |
Sprache: |
Chinesisch |
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Beteiligte Personen: |
Hao, Hui-fang [VerfasserIn] |
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Themen: |
DNA, Complementary |
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Anmerkungen: |
Date Completed 09.02.2009 Date Revised 27.11.2007 published: Print Citation Status MEDLINE |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM175358982 |
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100 | 1 | |a Hao, Hui-fang |e verfasserin |4 aut | |
245 | 1 | 0 | |a Cloning and construction of nucleic acid vaccine of FABP gene cDNA from Echinococcus granulosus |
264 | 1 | |c 2007 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
338 | |a Band |b nc |2 rdacarrier | ||
500 | |a Date Completed 09.02.2009 | ||
500 | |a Date Revised 27.11.2007 | ||
500 | |a published: Print | ||
500 | |a Citation Status MEDLINE | ||
520 | |a Specific primers were designed according to published nucleotide sequence of FABP (fatty acid binding protein) gene in the GenBank database. The kozak sequence (CCACC) was introduced at the upstream of initiator. The total RNA was extracted from protoscoleces of Echinococcus granulosus (Inner Mongol isolate). The FABP gene cDNA fragment was amplified by RT-PCR and cloned into pMD19-T vector for sequencing and analyzing. The cloned FABP gene cDNA was with 402bp. The ORF encoded 133 amino acids. The amplified cDNA fragment was subcloned into pCDNA3.1 (+) vector. The results showed that the nucleic acid vaccine candidate pcDNA-FABP-NM has been constructed | ||
650 | 4 | |a English Abstract | |
650 | 4 | |a Journal Article | |
650 | 4 | |a Research Support, Non-U.S. Gov't | |
650 | 7 | |a DNA, Complementary |2 NLM | |
650 | 7 | |a Fatty Acid-Binding Proteins |2 NLM | |
650 | 7 | |a Helminth Proteins |2 NLM | |
650 | 7 | |a Vaccines, DNA |2 NLM | |
700 | 1 | |a Wang, Zhi-gang |e verfasserin |4 aut | |
700 | 1 | |a Li, Zhi-wei |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases |d 1995 |g 25(2007), 4 vom: 19. Aug., Seite 352-4 |w (DE-627)NLM01284277X |x 1000-7423 |7 nnns |
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