Cloning and construction of nucleic acid vaccine of FABP gene cDNA from Echinococcus granulosus

Specific primers were designed according to published nucleotide sequence of FABP (fatty acid binding protein) gene in the GenBank database. The kozak sequence (CCACC) was introduced at the upstream of initiator. The total RNA was extracted from protoscoleces of Echinococcus granulosus (Inner Mongol isolate). The FABP gene cDNA fragment was amplified by RT-PCR and cloned into pMD19-T vector for sequencing and analyzing. The cloned FABP gene cDNA was with 402bp. The ORF encoded 133 amino acids. The amplified cDNA fragment was subcloned into pCDNA3.1 (+) vector. The results showed that the nucleic acid vaccine candidate pcDNA-FABP-NM has been constructed.

Medienart:

Artikel

Erscheinungsjahr:

2007

Erschienen:

2007

Enthalten in:

Zur Gesamtaufnahme - volume:25

Enthalten in:

Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases - 25(2007), 4 vom: 19. Aug., Seite 352-4

Sprache:

Chinesisch

Beteiligte Personen:

Hao, Hui-fang [VerfasserIn]
Wang, Zhi-gang [VerfasserIn]
Li, Zhi-wei [VerfasserIn]

Themen:

DNA, Complementary
English Abstract
Fatty Acid-Binding Proteins
Helminth Proteins
Journal Article
Research Support, Non-U.S. Gov't
Vaccines, DNA

Anmerkungen:

Date Completed 09.02.2009

Date Revised 27.11.2007

published: Print

Citation Status MEDLINE

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM175358982