Correlation between osmolarity and luminescence of symbiotic Vibrio fischeri strain ES114
Vibrio fischeri isolates from Euprymna scolopes are dim in culture but bright in the host. We found the luminescence of V. fischeri to be correlated with external osmolarity both in culture and in this symbiosis. Luminescence enhancement by osmolarity was independent of the lux promoter and unaffected by autoinducers or the level of lux expression, but the addition of an aldehyde substrate for luciferase raised the luminescence of cells grown at high and low osmolarities to the same high level. V. fischeri culture media have lower osmolarities than are typical in seawater or in cephalopods, partially accounting for the bacterium's low light output in culture.
Medienart: |
Artikel |
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Erscheinungsjahr: |
2004 |
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Erschienen: |
2004 |
Enthalten in: |
Zur Gesamtaufnahme - volume:186 |
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Enthalten in: |
Journal of bacteriology - 186(2004), 9 vom: 01. Mai, Seite 2906-8 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Stabb, Eric V [VerfasserIn] |
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Anmerkungen: |
Date Completed 20.05.2004 Date Revised 26.05.2021 published: Print Citation Status MEDLINE |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM147845424 |
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245 | 1 | 0 | |a Correlation between osmolarity and luminescence of symbiotic Vibrio fischeri strain ES114 |
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520 | |a Vibrio fischeri isolates from Euprymna scolopes are dim in culture but bright in the host. We found the luminescence of V. fischeri to be correlated with external osmolarity both in culture and in this symbiosis. Luminescence enhancement by osmolarity was independent of the lux promoter and unaffected by autoinducers or the level of lux expression, but the addition of an aldehyde substrate for luciferase raised the luminescence of cells grown at high and low osmolarities to the same high level. V. fischeri culture media have lower osmolarities than are typical in seawater or in cephalopods, partially accounting for the bacterium's low light output in culture | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Research Support, Non-U.S. Gov't | |
650 | 4 | |a Research Support, U.S. Gov't, P.H.S. | |
650 | 7 | |a Culture Media |2 NLM | |
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