Correlation between osmolarity and luminescence of symbiotic Vibrio fischeri strain ES114

Vibrio fischeri isolates from Euprymna scolopes are dim in culture but bright in the host. We found the luminescence of V. fischeri to be correlated with external osmolarity both in culture and in this symbiosis. Luminescence enhancement by osmolarity was independent of the lux promoter and unaffected by autoinducers or the level of lux expression, but the addition of an aldehyde substrate for luciferase raised the luminescence of cells grown at high and low osmolarities to the same high level. V. fischeri culture media have lower osmolarities than are typical in seawater or in cephalopods, partially accounting for the bacterium's low light output in culture.

Medienart:

Artikel

Erscheinungsjahr:

2004

Erschienen:

2004

Enthalten in:

Zur Gesamtaufnahme - volume:186

Enthalten in:

Journal of bacteriology - 186(2004), 9 vom: 01. Mai, Seite 2906-8

Sprache:

Englisch

Beteiligte Personen:

Stabb, Eric V [VerfasserIn]
Butler, Melissa S [VerfasserIn]
Adin, Dawn M [VerfasserIn]

Themen:

115038-68-1
451W47IQ8X
Culture Media
Journal Article
LuxR autoinducer binding proteins
Repressor Proteins
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.
Sodium Chloride
Trans-Activators

Anmerkungen:

Date Completed 20.05.2004

Date Revised 26.05.2021

published: Print

Citation Status MEDLINE

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM147845424