Affinity of Serum Natural Antibodies against Peptidoglycan Bacterial Cell Component with Adjuvant Activity
We studied affinity of natural antibodies of human serum against glucosaminylmuramyl dipeptide (GMDP), the epitope peptidoglycan bacterial cell wall component which carries adjuvant activity. Antibodies against GMDP were isolated from the blood sera of healthy donors using thermal extraction of antibodies from specific ligand on plastic. Determination of the dissociation constant (K(d)) showed equal K(d) in the serum and affinity-purified anti-GMDP-antibodies, i.e. extraction by this method led to the isolation of all subpopulations of antibodies in spite of their affinity. K(d) of serum, affinity-purified and monoclonal anti-GMDP-antibodies proved of low value - 10(-6) M, and according to this index anti-GMDP-antibodies may be classified between anti-protein and anti-carbohydrate antibodies.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
1997 |
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| Erschienen: |
1997 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:2 |
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| Enthalten in: |
Russian journal of immunology : RJI : official journal of Russian Society of Immunology - 2(1997), 3-4 vom: 10. Dez., Seite 177-182 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Kulakov, Alexandr V. [VerfasserIn] |
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| Themen: |
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| Anmerkungen: |
Date Revised 20.11.2019 published: Print Citation Status Publisher |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM124587992 |
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| 100 | 1 | |a Kulakov, Alexandr V. |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Affinity of Serum Natural Antibodies against Peptidoglycan Bacterial Cell Component with Adjuvant Activity |
| 264 | 1 | |c 1997 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
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| 500 | |a Date Revised 20.11.2019 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status Publisher | ||
| 520 | |a We studied affinity of natural antibodies of human serum against glucosaminylmuramyl dipeptide (GMDP), the epitope peptidoglycan bacterial cell wall component which carries adjuvant activity. Antibodies against GMDP were isolated from the blood sera of healthy donors using thermal extraction of antibodies from specific ligand on plastic. Determination of the dissociation constant (K(d)) showed equal K(d) in the serum and affinity-purified anti-GMDP-antibodies, i.e. extraction by this method led to the isolation of all subpopulations of antibodies in spite of their affinity. K(d) of serum, affinity-purified and monoclonal anti-GMDP-antibodies proved of low value - 10(-6) M, and according to this index anti-GMDP-antibodies may be classified between anti-protein and anti-carbohydrate antibodies | ||
| 650 | 4 | |a Journal Article | |
| 700 | 1 | |a Klimova, Svetlana V. |e verfasserin |4 aut | |
| 700 | 1 | |a Yarilin, Dmitry A. |e verfasserin |4 aut | |
| 700 | 1 | |a Rubtsova, Maja V. |e verfasserin |4 aut | |
| 700 | 1 | |a Pinegin, Boris V. |e verfasserin |4 aut | |
| 700 | 1 | |a Khaitov, Rakhim M. |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Russian journal of immunology : RJI : official journal of Russian Society of Immunology |d 1996 |g 2(1997), 3-4 vom: 10. Dez., Seite 177-182 |w (DE-627)NLM096531614 |x 1028-7221 |7 nnns |
| 773 | 1 | 8 | |g volume:2 |g year:1997 |g number:3-4 |g day:10 |g month:12 |g pages:177-182 |
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| 952 | |d 2 |j 1997 |e 3-4 |b 10 |c 12 |h 177-182 | ||