Characterization of novel breast carcinoma-associated BA46-derived peptides in HLA-A2.1/D(b)-beta2m transgenic mice

The human milk fat globule membrane protein BA46 (lactadherin) is highly overexpressed in human breast tumors, making it a potential target for tumor immunotherapy. We have identified BA46-derived peptides that contain the motif recognized by the MHC class I molecule HLA-A2.1 and that are processed and presented by human breast carcinoma cells. In mice lacking normal class I molecules but expressing an HLA-A2.1/D(b)-beta2 microglobulin single chain (HHD mice), three peptides elicited specific CTL activity. Two of these peptides also stimulated cytotoxic activity in peripheral blood lymphocytes from HLA-A2.1-positive breast carcinoma patients. Adoptive transfer of HHD-derived bulk CTLs to nude mice bearing human breast carcinoma transplants reduced tumor growth. These peptides therefore represent naturally processed BA46-derived CTL epitopes that can be used in peptide-based antitumor vaccines.

Medienart:

Artikel

Erscheinungsjahr:

2002

Erschienen:

2002

Enthalten in:

Zur Gesamtaufnahme - volume:110

Enthalten in:

The Journal of clinical investigation - 110(2002), 4 vom: 21. Aug., Seite 453-62

Sprache:

Englisch

Beteiligte Personen:

Carmon, Lior [VerfasserIn]
Bobilev-Priel, Irene [VerfasserIn]
Brenner, Baruch [VerfasserIn]
Bobilev, Dimitry [VerfasserIn]
Paz, Adrian [VerfasserIn]
Bar-Haim, Erez [VerfasserIn]
Tirosh, Boaz [VerfasserIn]
Klein, Tirza [VerfasserIn]
Fridkin, Mati [VerfasserIn]
Lemonnier, Francois [VerfasserIn]
Tzehoval, Esther [VerfasserIn]
Eisenbach, Lea [VerfasserIn]

Themen:

Antigens, Surface
Beta 2-Microglobulin
Cell Extracts
Epitopes
HLA-A2 Antigen
Journal Article
MFGE8 protein, human
Mfge8 protein, mouse
Milk Proteins
Neoplasm Proteins
Peptides
RNA, Neoplasm
Research Support, Non-U.S. Gov't

Anmerkungen:

Date Completed 06.09.2002

Date Revised 13.11.2018

published: Print

Citation Status MEDLINE

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM120493810