Determining the molar mass of a plasma substitute succinylated gelatin by size exclusion chromatography-multi-angle laser light scattering, sedimentation equilibrium and conventional size exclusion chromatography
The clinical effectiveness of succinylated gelatin as a plasma substitute depends strongly on its molar mass, determined conventionally by size exclusion chromatography (SEC). This study evaluates different SEC calibration standards in comparison with two independent "absolute" methods for determining the weight average molar mass (M(w)) of a succinylated gelatin sample. SEC calibrated using succinylated gelatin fractions correlated well with size exclusion chromatography-multi-angle laser light scattering (SEC-MALLS) and sedimentation equilibrium whereas SEC calibrated with unmodified gelatin, sodium polystyrene sulfonates or pullulans overestimated M(w) by over 20%. Universal calibration was equivocal. The problems associated with the preparation of succinylated gelatin fractions suggest that an absolute method such as SEC-MALLS may be a more suitable choice for determining the M(w) in succinylated gelatins.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2002 |
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| Erschienen: |
2002 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:957 |
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| Enthalten in: |
Journal of chromatography. A - 957(2002), 2 vom: 31. Mai, Seite 139-48 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Kaur, Manjit [VerfasserIn] |
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| Themen: |
39340-57-3 |
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| Anmerkungen: |
Date Completed 19.12.2002 Date Revised 20.07.2019 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM119823446 |
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| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Kaur, Manjit |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Determining the molar mass of a plasma substitute succinylated gelatin by size exclusion chromatography-multi-angle laser light scattering, sedimentation equilibrium and conventional size exclusion chromatography |
| 264 | 1 | |c 2002 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 19.12.2002 | ||
| 500 | |a Date Revised 20.07.2019 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a The clinical effectiveness of succinylated gelatin as a plasma substitute depends strongly on its molar mass, determined conventionally by size exclusion chromatography (SEC). This study evaluates different SEC calibration standards in comparison with two independent "absolute" methods for determining the weight average molar mass (M(w)) of a succinylated gelatin sample. SEC calibrated using succinylated gelatin fractions correlated well with size exclusion chromatography-multi-angle laser light scattering (SEC-MALLS) and sedimentation equilibrium whereas SEC calibrated with unmodified gelatin, sodium polystyrene sulfonates or pullulans overestimated M(w) by over 20%. Universal calibration was equivocal. The problems associated with the preparation of succinylated gelatin fractions suggest that an absolute method such as SEC-MALLS may be a more suitable choice for determining the M(w) in succinylated gelatins | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Plasma Substitutes |2 NLM | |
| 650 | 7 | |a Succinates |2 NLM | |
| 650 | 7 | |a succinylated gelatin |2 NLM | |
| 650 | 7 | |a 39340-57-3 |2 NLM | |
| 650 | 7 | |a Gelatin |2 NLM | |
| 650 | 7 | |a 9000-70-8 |2 NLM | |
| 700 | 1 | |a Jumel, Kornelia |e verfasserin |4 aut | |
| 700 | 1 | |a Hardie, Kim R |e verfasserin |4 aut | |
| 700 | 1 | |a Hardman, Andrea |e verfasserin |4 aut | |
| 700 | 1 | |a Meadows, John |e verfasserin |4 aut | |
| 700 | 1 | |a Melia, Colin D |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of chromatography. A |d 1993 |g 957(2002), 2 vom: 31. Mai, Seite 139-48 |w (DE-627)NLM074731416 |x 1873-3778 |7 nnns |
| 773 | 1 | 8 | |g volume:957 |g year:2002 |g number:2 |g day:31 |g month:05 |g pages:139-48 |
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| 952 | |d 957 |j 2002 |e 2 |b 31 |c 05 |h 139-48 | ||