Micro-injection-mediated hematopoietic stem cell gene therapy

Over the past decade, significant attention has been devoted to the development of viral vectors (i.e., retrovirus, lentivirus, adeno-associated virus) and conditions capable of transducing hematopoietic stem cells. After several years of disappointing results, recent reports in humans and other primates, most particularly the French report of successful treatment of X-linked severe combined immune deficiency (SCID) [1.], indicate that viral approaches will be successful in treating specific hematopoietic diseases. However, it is clear that alternate non-viral methods of gene delivery and genetic modification offer significant advantages, and may in fact be the only effective approach for treating certain blood diseases. In this review, we focus on glass needle-mediated micro-injection as a method for the delivery of genetic material into blood stem cells, with an emphasis on molecules capable of either compensating gene deletions/mutations or directly repairing gene mutations.

Medienart:

Artikel

Erscheinungsjahr:

2000

Erschienen:

2000

Enthalten in:

Zur Gesamtaufnahme - volume:2

Enthalten in:

Current opinion in molecular therapeutics - 2(2000), 4 vom: 15. Aug., Seite 412-9

Sprache:

Englisch

Beteiligte Personen:

Davis, B R [VerfasserIn]
Brown, D B [VerfasserIn]
Prokopishyn, N L [VerfasserIn]
Yannariello-Brown, J [VerfasserIn]

Themen:

63231-63-0
9007-49-2
DNA
Journal Article
RNA
Review

Anmerkungen:

Date Completed 12.04.2001

Date Revised 20.11.2014

published: Print

Citation Status MEDLINE

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM111605237