Production of a functional single chain antibody attached to the surface of a plant virus

A potato virus X (PVX) vector was used to express a single chain antibody fragment (scFv) against the herbicide diuron, as a fusion to the viral coat protein. The modified virus accumulated in inoculated Nicotiana clevelandii plants and assembled to give virus particles carrying the antibody fragment. Electron microscopy was used to show that virus particles from infected leaf sap were specifically trapped on grids coated with a diuron-BSA conjugate. The results demonstrate that the PVX vector can be used as a presentation system for functional scFv.

Medienart:

Artikel

Erscheinungsjahr:

1998

Erschienen:

1998

Enthalten in:

Zur Gesamtaufnahme - volume:441

Enthalten in:

FEBS letters - 441(1998), 3 vom: 28. Dez., Seite 379-82

Sprache:

Englisch

Beteiligte Personen:

Smolenska, L [VerfasserIn]
Roberts, I M [VerfasserIn]
Learmonth, D [VerfasserIn]
Porter, A J [VerfasserIn]
Harris, W J [VerfasserIn]
Wilson, T M [VerfasserIn]
Santa Cruz, S [VerfasserIn]

Themen:

9I3SDS92WY
Diuron
Herbicides
Immunoglobulin Fragments
Journal Article
Recombinant Proteins
Research Support, Non-U.S. Gov't

Anmerkungen:

Date Completed 05.02.1999

Date Revised 13.12.2023

published: Print

Citation Status MEDLINE

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM098272152