Production of a functional single chain antibody attached to the surface of a plant virus
A potato virus X (PVX) vector was used to express a single chain antibody fragment (scFv) against the herbicide diuron, as a fusion to the viral coat protein. The modified virus accumulated in inoculated Nicotiana clevelandii plants and assembled to give virus particles carrying the antibody fragment. Electron microscopy was used to show that virus particles from infected leaf sap were specifically trapped on grids coated with a diuron-BSA conjugate. The results demonstrate that the PVX vector can be used as a presentation system for functional scFv.
Medienart: |
Artikel |
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Erscheinungsjahr: |
1998 |
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Erschienen: |
1998 |
Enthalten in: |
Zur Gesamtaufnahme - volume:441 |
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Enthalten in: |
FEBS letters - 441(1998), 3 vom: 28. Dez., Seite 379-82 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Smolenska, L [VerfasserIn] |
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Themen: |
9I3SDS92WY |
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Anmerkungen: |
Date Completed 05.02.1999 Date Revised 13.12.2023 published: Print Citation Status MEDLINE |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM098272152 |
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245 | 1 | 0 | |a Production of a functional single chain antibody attached to the surface of a plant virus |
264 | 1 | |c 1998 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
338 | |a Band |b nc |2 rdacarrier | ||
500 | |a Date Completed 05.02.1999 | ||
500 | |a Date Revised 13.12.2023 | ||
500 | |a published: Print | ||
500 | |a Citation Status MEDLINE | ||
520 | |a A potato virus X (PVX) vector was used to express a single chain antibody fragment (scFv) against the herbicide diuron, as a fusion to the viral coat protein. The modified virus accumulated in inoculated Nicotiana clevelandii plants and assembled to give virus particles carrying the antibody fragment. Electron microscopy was used to show that virus particles from infected leaf sap were specifically trapped on grids coated with a diuron-BSA conjugate. The results demonstrate that the PVX vector can be used as a presentation system for functional scFv | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Research Support, Non-U.S. Gov't | |
650 | 7 | |a Herbicides |2 NLM | |
650 | 7 | |a Immunoglobulin Fragments |2 NLM | |
650 | 7 | |a Recombinant Proteins |2 NLM | |
650 | 7 | |a Diuron |2 NLM | |
650 | 7 | |a 9I3SDS92WY |2 NLM | |
700 | 1 | |a Roberts, I M |e verfasserin |4 aut | |
700 | 1 | |a Learmonth, D |e verfasserin |4 aut | |
700 | 1 | |a Porter, A J |e verfasserin |4 aut | |
700 | 1 | |a Harris, W J |e verfasserin |4 aut | |
700 | 1 | |a Wilson, T M |e verfasserin |4 aut | |
700 | 1 | |a Santa Cruz, S |e verfasserin |4 aut | |
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