Human corneal epithelial basement membrane and integrin alterations in diabetes and diabetic retinopathy
Corneas of diabetic patients have abnormal healing and epithelial adhesion, which may be due to alterations of the corneal extracellular matrix (ECM) and basement membrane (BM). To identify such alterations, various ECM and BM components and integrin receptors were studied by immunofluorescence on sections of normal and diabetic human corneas. Age-matched corneas from 15 normal subjects, 10 diabetics without diabetic retinopathy (DR), and 12 diabetics with DR were used. In DR corneas, the composition of the central epithelial BM was markedly altered, compared to normal or non-DR diabetic corneas. In most cases the staining for entactin/nidogen and for chains of laminin-1 (alpha1beta1gamma1) and laminin-10 (alpha5beta1gamma1 was very weak, discontinuous, or absent over large areas. Other BM components displayed less frequent changes. The staining for alpha3beta1 (VLA-3) laminin binding integrin was also weak and discontinuous in DR corneal epithelium. Components of stromal ECM remained unchanged even in DR corneas. Therefore, distinct changes were identified in the composition of the epithelial BM in DR corneas. They may be due to increased degradation or decreased synthesis of BM components and related integrins. These alterations may directly contribute to the epithelial adhesion and wound healing abnormalities found in diabetic corneas.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
1998 |
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| Erschienen: |
1998 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:46 |
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| Enthalten in: |
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society - 46(1998), 9 vom: 17. Sept., Seite 1033-41 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Ljubimov, A V [VerfasserIn] |
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| Themen: |
Extracellular Matrix Proteins |
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| Anmerkungen: |
Date Completed 22.09.1998 Date Revised 14.02.2017 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM096453346 |
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| 100 | 1 | |a Ljubimov, A V |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Human corneal epithelial basement membrane and integrin alterations in diabetes and diabetic retinopathy |
| 264 | 1 | |c 1998 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 22.09.1998 | ||
| 500 | |a Date Revised 14.02.2017 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Corneas of diabetic patients have abnormal healing and epithelial adhesion, which may be due to alterations of the corneal extracellular matrix (ECM) and basement membrane (BM). To identify such alterations, various ECM and BM components and integrin receptors were studied by immunofluorescence on sections of normal and diabetic human corneas. Age-matched corneas from 15 normal subjects, 10 diabetics without diabetic retinopathy (DR), and 12 diabetics with DR were used. In DR corneas, the composition of the central epithelial BM was markedly altered, compared to normal or non-DR diabetic corneas. In most cases the staining for entactin/nidogen and for chains of laminin-1 (alpha1beta1gamma1) and laminin-10 (alpha5beta1gamma1 was very weak, discontinuous, or absent over large areas. Other BM components displayed less frequent changes. The staining for alpha3beta1 (VLA-3) laminin binding integrin was also weak and discontinuous in DR corneal epithelium. Components of stromal ECM remained unchanged even in DR corneas. Therefore, distinct changes were identified in the composition of the epithelial BM in DR corneas. They may be due to increased degradation or decreased synthesis of BM components and related integrins. These alterations may directly contribute to the epithelial adhesion and wound healing abnormalities found in diabetic corneas | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Extracellular Matrix Proteins |2 NLM | |
| 650 | 7 | |a Integrins |2 NLM | |
| 650 | 7 | |a Laminin |2 NLM | |
| 700 | 1 | |a Huang, Z S |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, G H |e verfasserin |4 aut | |
| 700 | 1 | |a Burgeson, R E |e verfasserin |4 aut | |
| 700 | 1 | |a Gullberg, D |e verfasserin |4 aut | |
| 700 | 1 | |a Miner, J H |e verfasserin |4 aut | |
| 700 | 1 | |a Ninomiya, Y |e verfasserin |4 aut | |
| 700 | 1 | |a Sado, Y |e verfasserin |4 aut | |
| 700 | 1 | |a Kenney, M C |e verfasserin |4 aut | |
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