A major DNA binding protein encoded by BALF2 open reading frame of Epstein-Barr virus (EBV) forms a complex with other EBV DNA-binding proteins : DNAase, EA-D, and DNA polymerase
A major 135-kDa DNA binding protein (mDBP) encoded by the BALF2 open reading frame of Epstein-Barr Virus (EBV) is known to be an essential protein for the induction of the lytic cycle. The present investigation was carried out to know whether this protein forms a complex in vivo with other viral DNA binding proteins (DBP) involved in DNA replication: DNA polymerase, EA-D (diffused early antigen), and DNAase. Immunoprecipitation assays followed by mono- and two-dimensional electrophoresis showed that mDBP forms a complex with these three DBP. Other complexes were also found such as EA-D/DNAase, DNA polymerase/DNAase, and DNA polymerase/EA-D. The complexed forms already exist in the early stage of EBV cycle before DNA synthesis is induced in the EBV producer P3HR-1 cell line. The exonuclease activity encoded by DNAase was found to be inhibited when this enzyme complexed with mDBP, while the EBV DNA polymerase retained its activity in the complexed form with mDBP. Our results suggest that these complexes already present before DNA synthesis are necessary for EBV DNA synthesis.
Medienart: |
Artikel |
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Erscheinungsjahr: |
1997 |
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Erschienen: |
1997 |
Enthalten in: |
Zur Gesamtaufnahme - volume:239 |
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Enthalten in: |
Virology - 239(1997), 2 vom: 22. Dez., Seite 285-95 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Zeng, Y [VerfasserIn] |
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Anmerkungen: |
Date Completed 12.02.1998 Date Revised 15.11.2006 published: Print Citation Status MEDLINE |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM093780508 |
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041 | |a eng | ||
100 | 1 | |a Zeng, Y |e verfasserin |4 aut | |
245 | 1 | 2 | |a A major DNA binding protein encoded by BALF2 open reading frame of Epstein-Barr virus (EBV) forms a complex with other EBV DNA-binding proteins |b DNAase, EA-D, and DNA polymerase |
264 | 1 | |c 1997 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
338 | |a Band |b nc |2 rdacarrier | ||
500 | |a Date Completed 12.02.1998 | ||
500 | |a Date Revised 15.11.2006 | ||
500 | |a published: Print | ||
500 | |a Citation Status MEDLINE | ||
520 | |a A major 135-kDa DNA binding protein (mDBP) encoded by the BALF2 open reading frame of Epstein-Barr Virus (EBV) is known to be an essential protein for the induction of the lytic cycle. The present investigation was carried out to know whether this protein forms a complex in vivo with other viral DNA binding proteins (DBP) involved in DNA replication: DNA polymerase, EA-D (diffused early antigen), and DNAase. Immunoprecipitation assays followed by mono- and two-dimensional electrophoresis showed that mDBP forms a complex with these three DBP. Other complexes were also found such as EA-D/DNAase, DNA polymerase/DNAase, and DNA polymerase/EA-D. The complexed forms already exist in the early stage of EBV cycle before DNA synthesis is induced in the EBV producer P3HR-1 cell line. The exonuclease activity encoded by DNAase was found to be inhibited when this enzyme complexed with mDBP, while the EBV DNA polymerase retained its activity in the complexed form with mDBP. Our results suggest that these complexes already present before DNA synthesis are necessary for EBV DNA synthesis | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Research Support, Non-U.S. Gov't | |
650 | 7 | |a Antigens, Viral |2 NLM | |
650 | 7 | |a BALF2 protein, Human herpesvirus 4 |2 NLM | |
650 | 7 | |a DNA, Viral |2 NLM | |
650 | 7 | |a DNA-Binding Proteins |2 NLM | |
650 | 7 | |a Epstein-Barr virus early antigen diffuse component |2 NLM | |
650 | 7 | |a Multienzyme Complexes |2 NLM | |
650 | 7 | |a Viral Proteins |2 NLM | |
650 | 7 | |a BALF5 protein, Epstein-barr virus |2 NLM | |
650 | 7 | |a EC 2.7.7.7 |2 NLM | |
650 | 7 | |a DNA-Directed DNA Polymerase |2 NLM | |
650 | 7 | |a EC 2.7.7.7 |2 NLM | |
650 | 7 | |a Deoxyribonucleases |2 NLM | |
650 | 7 | |a EC 3.1.- |2 NLM | |
650 | 7 | |a deoxyribonuclease, Epstein-Barr virus |2 NLM | |
650 | 7 | |a EC 3.1.11.- |2 NLM | |
700 | 1 | |a Middeldorp, J |e verfasserin |4 aut | |
700 | 1 | |a Madjar, J J |e verfasserin |4 aut | |
700 | 1 | |a Ooka, T |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Virology |d 1955 |g 239(1997), 2 vom: 22. Dez., Seite 285-95 |w (DE-627)NLM000011347 |x 1096-0341 |7 nnns |
773 | 1 | 8 | |g volume:239 |g year:1997 |g number:2 |g day:22 |g month:12 |g pages:285-95 |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 239 |j 1997 |e 2 |b 22 |c 12 |h 285-95 |