Structure, organization and expression of the mouse ornithine decarboxylase antizyme gene
Ornithine decarboxylase antizyme is a protein that participates in the regulation of cellular polyamine levels. In this study we have isolated and sequenced the mouse gene encoding antizyme protein. Transfection of various cell lines with a 5.5 kb genomic fragment containing the antizyme locus resulted in the production of a 29 kDa antizyme protein, confirming that this locus contained a functional gene. Comparison of the mouse gene with the corresponding rat gene [Miyazaki, Matsufuji and Hayashi, (1992) Gene 113, 191-197] revealed an identical exon/intron organization and high level of nucleotide sequence conservation that was 89% for the entire transcription unit. Protein-coding regions of the two genes exhibited 97% nucleotide sequence identity and there were only four amino acid differences between the 227-residue antizyme protein sequences of the mouse and rat. The promoter of the antizyme gene was functional in mouse (N2A and NIH/3T3) and hamster (CHO) cell lines. The presence of 0.1 mM spermidine in culture medium increased the amount of immunoreactive antizyme protein in cells transfected with the antizyme gene or antizyme cDNA, possibly owing to facilitated frameshifting in the translation of antizyme mRNA. Recombinant antizyme protein was also produced in Escherichia coli and used to raise specific polyclonal antibodies in rabbits and to devise immunological methods for the measurement of antizyme concentration.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
1997 |
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| Erschienen: |
1997 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:324 ( Pt 3) |
|---|---|
| Enthalten in: |
The Biochemical journal - 324 ( Pt 3)(1997) vom: 15. Juni, Seite 807-13 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Kankare, K [VerfasserIn] |
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| Themen: |
DNA, Complementary |
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| Anmerkungen: |
Date Completed 28.07.1997 Date Revised 01.05.2019 published: Print GENBANK: U84291 Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM091650739 |
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| 100 | 1 | |a Kankare, K |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Structure, organization and expression of the mouse ornithine decarboxylase antizyme gene |
| 264 | 1 | |c 1997 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 28.07.1997 | ||
| 500 | |a Date Revised 01.05.2019 | ||
| 500 | |a published: Print | ||
| 500 | |a GENBANK: U84291 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Ornithine decarboxylase antizyme is a protein that participates in the regulation of cellular polyamine levels. In this study we have isolated and sequenced the mouse gene encoding antizyme protein. Transfection of various cell lines with a 5.5 kb genomic fragment containing the antizyme locus resulted in the production of a 29 kDa antizyme protein, confirming that this locus contained a functional gene. Comparison of the mouse gene with the corresponding rat gene [Miyazaki, Matsufuji and Hayashi, (1992) Gene 113, 191-197] revealed an identical exon/intron organization and high level of nucleotide sequence conservation that was 89% for the entire transcription unit. Protein-coding regions of the two genes exhibited 97% nucleotide sequence identity and there were only four amino acid differences between the 227-residue antizyme protein sequences of the mouse and rat. The promoter of the antizyme gene was functional in mouse (N2A and NIH/3T3) and hamster (CHO) cell lines. The presence of 0.1 mM spermidine in culture medium increased the amount of immunoreactive antizyme protein in cells transfected with the antizyme gene or antizyme cDNA, possibly owing to facilitated frameshifting in the translation of antizyme mRNA. Recombinant antizyme protein was also produced in Escherichia coli and used to raise specific polyclonal antibodies in rabbits and to devise immunological methods for the measurement of antizyme concentration | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a DNA, Complementary |2 NLM | |
| 650 | 7 | |a Ornithine Decarboxylase Inhibitors |2 NLM | |
| 650 | 7 | |a Proteins |2 NLM | |
| 650 | 7 | |a ornithine decarboxylase antizyme |2 NLM | |
| 700 | 1 | |a Uusi-Oukari, M |e verfasserin |4 aut | |
| 700 | 1 | |a Jänne, O A |e verfasserin |4 aut | |
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