Inhibitory kinetic studies on rat hepatic and renal succinate dehydrogenase with guanidine
Guanidine-induced alterations in substrate dependent kinetics of hepatic and renal succinate dehydrogenase (SDH) have been investigated under in vitro conditions. Guanidine hydrochloride (GuHCl) induced a mixed type of inhibition by decreasing the maximal velocity (Vmax) and increasing the Michaelis-Menten constant (Km). The competitive (Ki) and non-competitive (Ki) inhibitory constants were calculated. The values showed that the inhibitory influence of GuHCl is more due to decreased enzyme substrate affinity rather than reduction in the active site density of the enzyme as revealed by low Ki values.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
1997 |
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| Erschienen: |
1997 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:11 |
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| Enthalten in: |
Journal of enzyme inhibition - 11(1997), 4 vom: 15. Feb., Seite 293-8 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Rao, S V [VerfasserIn] |
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| Themen: |
Comparative Study |
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| Anmerkungen: |
Date Completed 07.08.1997 Date Revised 02.11.2019 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM091631157 |
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| 100 | 1 | |a Rao, S V |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Inhibitory kinetic studies on rat hepatic and renal succinate dehydrogenase with guanidine |
| 264 | 1 | |c 1997 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 07.08.1997 | ||
| 500 | |a Date Revised 02.11.2019 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Guanidine-induced alterations in substrate dependent kinetics of hepatic and renal succinate dehydrogenase (SDH) have been investigated under in vitro conditions. Guanidine hydrochloride (GuHCl) induced a mixed type of inhibition by decreasing the maximal velocity (Vmax) and increasing the Michaelis-Menten constant (Km). The competitive (Ki) and non-competitive (Ki) inhibitory constants were calculated. The values showed that the inhibitory influence of GuHCl is more due to decreased enzyme substrate affinity rather than reduction in the active site density of the enzyme as revealed by low Ki values | ||
| 650 | 4 | |a Comparative Study | |
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Enzyme Inhibitors |2 NLM | |
| 650 | 7 | |a Guanidines |2 NLM | |
| 650 | 7 | |a Succinate Dehydrogenase |2 NLM | |
| 650 | 7 | |a EC 1.3.99.1 |2 NLM | |
| 650 | 7 | |a Guanidine |2 NLM | |
| 650 | 7 | |a JU58VJ6Y3B |2 NLM | |
| 700 | 1 | |a Sriram, K I |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of enzyme inhibition |d 1989 |g 11(1997), 4 vom: 15. Feb., Seite 293-8 |w (DE-627)NLM012649945 |x 8755-5093 |7 nnns |
| 773 | 1 | 8 | |g volume:11 |g year:1997 |g number:4 |g day:15 |g month:02 |g pages:293-8 |
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| 952 | |d 11 |j 1997 |e 4 |b 15 |c 02 |h 293-8 | ||