Culture of rat mesenteric arteriolar smooth muscle cells : effects of platelet-derived growth factor, angiotensin, and nitric oxide on growth
We cultured smooth muscle cells as explants from rat mesenteric arterioles (40-200 microns in diameter) obtained by injecting a suspension of iron oxide intraarterially and magnetically separating the arterioles after collagenase digestion of adventitial tissue. In third-passaged cells we ascertained smooth muscle purity of > 98% by characteristic morphology, contraction responses, and specific immunofluorescence staining. Treatment of growth-arrested (in 0.4% fetal calf serum) cells with platelet-derived growth factor (0.3-7.5 nM) or angiotensin II (0.001-1000 nM) induced 3H-thymidine incorporation and cell proliferation in a dose-dependent manner (P < 0.01). S-nitroso-N acetylpenicillamine (0.05-0.5 mM), a nitric oxide-generating compound, inhibited 10% fetal calf serum-induced 3H-thymidine incorporation (P < 0.05) and cell proliferation (P < 0.01). The antimitogenic effect of S-nitroso-N-acetylpenicillamine was significantly reduced by hemoglobin and potentiated by superoxide dismutase (P < 0.01). In addition to a new technique for culturing mesenteric arteriolar smooth muscle cells, these findings provide evidence that platelet-derived growth factor, angiotensin II, and nitric oxide may be involved in their growth control.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
1994 |
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| Erschienen: |
1994 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:275 |
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| Enthalten in: |
Cell and tissue research - 275(1994), 1 vom: 01. Jan., Seite 133-41 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Dubey, R K [VerfasserIn] |
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| Anmerkungen: |
Date Completed 04.04.1994 Date Revised 20.07.2019 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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NLM080939090 |
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| 100 | 1 | |a Dubey, R K |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Culture of rat mesenteric arteriolar smooth muscle cells |b effects of platelet-derived growth factor, angiotensin, and nitric oxide on growth |
| 264 | 1 | |c 1994 | |
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| 500 | |a Date Completed 04.04.1994 | ||
| 500 | |a Date Revised 20.07.2019 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a We cultured smooth muscle cells as explants from rat mesenteric arterioles (40-200 microns in diameter) obtained by injecting a suspension of iron oxide intraarterially and magnetically separating the arterioles after collagenase digestion of adventitial tissue. In third-passaged cells we ascertained smooth muscle purity of > 98% by characteristic morphology, contraction responses, and specific immunofluorescence staining. Treatment of growth-arrested (in 0.4% fetal calf serum) cells with platelet-derived growth factor (0.3-7.5 nM) or angiotensin II (0.001-1000 nM) induced 3H-thymidine incorporation and cell proliferation in a dose-dependent manner (P < 0.01). S-nitroso-N acetylpenicillamine (0.05-0.5 mM), a nitric oxide-generating compound, inhibited 10% fetal calf serum-induced 3H-thymidine incorporation (P < 0.05) and cell proliferation (P < 0.01). The antimitogenic effect of S-nitroso-N-acetylpenicillamine was significantly reduced by hemoglobin and potentiated by superoxide dismutase (P < 0.01). In addition to a new technique for culturing mesenteric arteriolar smooth muscle cells, these findings provide evidence that platelet-derived growth factor, angiotensin II, and nitric oxide may be involved in their growth control | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Research Support, U.S. Gov't, P.H.S. | |
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| 650 | 7 | |a Platelet-Derived Growth Factor |2 NLM | |
| 650 | 7 | |a Angiotensin II |2 NLM | |
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| 700 | 1 | |a Overbeck, H W |e verfasserin |4 aut | |
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