Enkephalin metabolism by microglial aminopeptidase N (CD13)
Rat microglia in culture showed a high capacity to degrade neuropeptides compared with other glial cells. Leu-enkephalin was readily hydrolyzed to free tyrosine and Gly-Gly-Phe-Leu. Inhibition experiments and immunostaining revealed that aminopeptidase N (CD13) on the surface of microglia was responsible for enkephalin cleavage. Endopeptidase-24.11 ("enkephalinase"), angiotensin-converting enzyme, or carboxypeptidases could not be detected on microglia. Aminopeptidase N activity in microglia was considerably higher than in rat peripheral monocytes and macrophages, which both also exhibited low endopeptidase 24.11 activities. Activity of aminopeptidase N was upregulated by culture of microglia on astrocytes and down-regulated by exposure of microglia to lipopolysaccharide. The occurrence of aminopeptidase N on microglia is in line with the view that they originate from the monocytic lineage.
| Medienart: |
Artikel |
|---|
| Erscheinungsjahr: |
1995 |
|---|---|
| Erschienen: |
1995 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:64 |
|---|---|
| Enthalten in: |
Journal of neurochemistry - 64(1995), 4 vom: 22. Apr., Seite 1841-7 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Lucius, R [VerfasserIn] |
|---|
| Themen: |
CD13 Antigens |
|---|
| Anmerkungen: |
Date Completed 18.04.1995 Date Revised 30.06.2019 published: Print Citation Status MEDLINE |
|---|
| Förderinstitution / Projekttitel: |
|
|---|
| PPN (Katalog-ID): |
NLM075104776 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM075104776 | ||
| 003 | DE-627 | ||
| 005 | 20231222024254.0 | ||
| 007 | tu | ||
| 008 | 231222s1995 xx ||||| 00| ||eng c | ||
| 028 | 5 | 2 | |a pubmed24n0251.xml |
| 035 | |a (DE-627)NLM075104776 | ||
| 035 | |a (NLM)7534337 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Lucius, R |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Enkephalin metabolism by microglial aminopeptidase N (CD13) |
| 264 | 1 | |c 1995 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 18.04.1995 | ||
| 500 | |a Date Revised 30.06.2019 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Rat microglia in culture showed a high capacity to degrade neuropeptides compared with other glial cells. Leu-enkephalin was readily hydrolyzed to free tyrosine and Gly-Gly-Phe-Leu. Inhibition experiments and immunostaining revealed that aminopeptidase N (CD13) on the surface of microglia was responsible for enkephalin cleavage. Endopeptidase-24.11 ("enkephalinase"), angiotensin-converting enzyme, or carboxypeptidases could not be detected on microglia. Aminopeptidase N activity in microglia was considerably higher than in rat peripheral monocytes and macrophages, which both also exhibited low endopeptidase 24.11 activities. Activity of aminopeptidase N was upregulated by culture of microglia on astrocytes and down-regulated by exposure of microglia to lipopolysaccharide. The occurrence of aminopeptidase N on microglia is in line with the view that they originate from the monocytic lineage | ||
| 650 | 4 | |a Comparative Study | |
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Enkephalins |2 NLM | |
| 650 | 7 | |a CD13 Antigens |2 NLM | |
| 650 | 7 | |a EC 3.4.11.2 |2 NLM | |
| 700 | 1 | |a Sievers, J |e verfasserin |4 aut | |
| 700 | 1 | |a Mentlein, R |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of neurochemistry |d 1956 |g 64(1995), 4 vom: 22. Apr., Seite 1841-7 |w (DE-627)NLM000006211 |x 1471-4159 |7 nnns |
| 773 | 1 | 8 | |g volume:64 |g year:1995 |g number:4 |g day:22 |g month:04 |g pages:1841-7 |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 64 |j 1995 |e 4 |b 22 |c 04 |h 1841-7 | ||