Characterization of the calcium influx induced by depolarization of guinea pig cochlear spiral ganglion cells
We examined dynamic changes in intracellular calcium ion concentrations ([Ca2+]i) in isolated cochlear spiral ganglion cells (SGCs) of the guinea pig using digital imaging microscopy and the Ca(2+)-sensitive fluorescence dye fura-2. [Ca2+]i in SGCs was 83 +/- 22 nM (n = 50, means +/- SD) in cell somata at the resting state. Reversible increases in [Ca2+]i were elicited during membrane depolarization by high K+ (150 mM). This increase in [Ca2+]i was not observed under conditions of depolarization in Ca(2+)-free medium containing 1 mM EGTA. In addition, these increases in [Ca2+]i were sensitive to L-type calcium channel ligands, viz., antagonized by nifedipine (50 microM), verapamil (10 microM) and enhanced by BAY K 8644 (1 microM). These observations suggest that increases in [Ca2+]i of cochlear SGCs induced by high K+ are due to an influx of extracellular Ca2+, probably through voltage-gated L-type calcium channels.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
1994 |
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| Erschienen: |
1994 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:56 |
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| Enthalten in: |
ORL; journal for oto-rhino-laryngology and its related specialties - 56(1994), 3 vom: 11. Mai, Seite 125-9 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Han, D Y [VerfasserIn] |
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| Anmerkungen: |
Date Completed 07.07.1994 Date Revised 16.02.2018 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM074915649 |
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| 100 | 1 | |a Han, D Y |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Characterization of the calcium influx induced by depolarization of guinea pig cochlear spiral ganglion cells |
| 264 | 1 | |c 1994 | |
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| 500 | |a Date Completed 07.07.1994 | ||
| 500 | |a Date Revised 16.02.2018 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a We examined dynamic changes in intracellular calcium ion concentrations ([Ca2+]i) in isolated cochlear spiral ganglion cells (SGCs) of the guinea pig using digital imaging microscopy and the Ca(2+)-sensitive fluorescence dye fura-2. [Ca2+]i in SGCs was 83 +/- 22 nM (n = 50, means +/- SD) in cell somata at the resting state. Reversible increases in [Ca2+]i were elicited during membrane depolarization by high K+ (150 mM). This increase in [Ca2+]i was not observed under conditions of depolarization in Ca(2+)-free medium containing 1 mM EGTA. In addition, these increases in [Ca2+]i were sensitive to L-type calcium channel ligands, viz., antagonized by nifedipine (50 microM), verapamil (10 microM) and enhanced by BAY K 8644 (1 microM). These observations suggest that increases in [Ca2+]i of cochlear SGCs induced by high K+ are due to an influx of extracellular Ca2+, probably through voltage-gated L-type calcium channels | ||
| 650 | 4 | |a Journal Article | |
| 650 | 7 | |a Calcium Channels |2 NLM | |
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| 700 | 1 | |a Harada, N |e verfasserin |4 aut | |
| 700 | 1 | |a Tomoda, K |e verfasserin |4 aut | |
| 700 | 1 | |a Yamashita, T |e verfasserin |4 aut | |
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