Pertussis toxin non-sensitive G protein mediates cholinergic stimulation for secretion of pancreastatin and somatostatin from QGP-1N cells
To clarify the possible role of a guanine nucleotide-binding protein (G-protein) in the signal transducing system activated by carbachol, actions of carbachol on human pancreastatin producing cell line (QGP-1N) were compared with those of fluoride, a well-known activator of stimulatory (Gs) or inhibitory (Gi) G protein. 10(-5) M of carbachol as well as 20 mM of NaF stimulated secretion of pancreastatin and somatostatin and intracellular Ca2+ mobilization. These secretion and Ca2+ mobilization were not modified by pertussis toxin, an inhibitor of Gi protein. These results suggest that pancreastatin and somatostatin secretions from QGP-1N are regulated by acetylcholine through a muscarinic receptor coupled to the activation of polyphosphoinositide breakdown by a G protein, which appears to be fluoride sensitive but is other than a Gi-like protein.
Medienart: |
Artikel |
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Erscheinungsjahr: |
1992 |
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Erschienen: |
1992 |
Enthalten in: |
Zur Gesamtaufnahme - volume:37 |
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Enthalten in: |
Regulatory peptides - 37(1992), 1 vom: 02. Jan., Seite 1-7 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Funakoshi, A [VerfasserIn] |
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Anmerkungen: |
Date Completed 12.06.1992 Date Revised 27.08.2019 published: Print Citation Status MEDLINE |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM013314238 |
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100 | 1 | |a Funakoshi, A |e verfasserin |4 aut | |
245 | 1 | 0 | |a Pertussis toxin non-sensitive G protein mediates cholinergic stimulation for secretion of pancreastatin and somatostatin from QGP-1N cells |
264 | 1 | |c 1992 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
338 | |a Band |b nc |2 rdacarrier | ||
500 | |a Date Completed 12.06.1992 | ||
500 | |a Date Revised 27.08.2019 | ||
500 | |a published: Print | ||
500 | |a Citation Status MEDLINE | ||
520 | |a To clarify the possible role of a guanine nucleotide-binding protein (G-protein) in the signal transducing system activated by carbachol, actions of carbachol on human pancreastatin producing cell line (QGP-1N) were compared with those of fluoride, a well-known activator of stimulatory (Gs) or inhibitory (Gi) G protein. 10(-5) M of carbachol as well as 20 mM of NaF stimulated secretion of pancreastatin and somatostatin and intracellular Ca2+ mobilization. These secretion and Ca2+ mobilization were not modified by pertussis toxin, an inhibitor of Gi protein. These results suggest that pancreastatin and somatostatin secretions from QGP-1N are regulated by acetylcholine through a muscarinic receptor coupled to the activation of polyphosphoinositide breakdown by a G protein, which appears to be fluoride sensitive but is other than a Gi-like protein | ||
650 | 4 | |a Journal Article | |
650 | 7 | |a Chromogranin A |2 NLM | |
650 | 7 | |a Pancreatic Hormones |2 NLM | |
650 | 7 | |a Virulence Factors, Bordetella |2 NLM | |
650 | 7 | |a pancreastatin |2 NLM | |
650 | 7 | |a 106477-83-2 |2 NLM | |
650 | 7 | |a Somatostatin |2 NLM | |
650 | 7 | |a 51110-01-1 |2 NLM | |
650 | 7 | |a Carbachol |2 NLM | |
650 | 7 | |a 8Y164V895Y |2 NLM | |
650 | 7 | |a Sodium Fluoride |2 NLM | |
650 | 7 | |a 8ZYQ1474W7 |2 NLM | |
650 | 7 | |a Pertussis Toxin |2 NLM | |
650 | 7 | |a EC 2.4.2.31 |2 NLM | |
650 | 7 | |a GTP-Binding Proteins |2 NLM | |
650 | 7 | |a EC 3.6.1.- |2 NLM | |
650 | 7 | |a Calcium |2 NLM | |
650 | 7 | |a SY7Q814VUP |2 NLM | |
700 | 1 | |a Tateishi, K |e verfasserin |4 aut | |
700 | 1 | |a Tsuru, M |e verfasserin |4 aut | |
700 | 1 | |a Kono, A |e verfasserin |4 aut | |
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