Pertussis toxin non-sensitive G protein mediates cholinergic stimulation for secretion of pancreastatin and somatostatin from QGP-1N cells

To clarify the possible role of a guanine nucleotide-binding protein (G-protein) in the signal transducing system activated by carbachol, actions of carbachol on human pancreastatin producing cell line (QGP-1N) were compared with those of fluoride, a well-known activator of stimulatory (Gs) or inhibitory (Gi) G protein. 10(-5) M of carbachol as well as 20 mM of NaF stimulated secretion of pancreastatin and somatostatin and intracellular Ca2+ mobilization. These secretion and Ca2+ mobilization were not modified by pertussis toxin, an inhibitor of Gi protein. These results suggest that pancreastatin and somatostatin secretions from QGP-1N are regulated by acetylcholine through a muscarinic receptor coupled to the activation of polyphosphoinositide breakdown by a G protein, which appears to be fluoride sensitive but is other than a Gi-like protein.

Medienart:

Artikel

Erscheinungsjahr:

1992

Erschienen:

1992

Enthalten in:

Zur Gesamtaufnahme - volume:37

Enthalten in:

Regulatory peptides - 37(1992), 1 vom: 02. Jan., Seite 1-7

Sprache:

Englisch

Beteiligte Personen:

Funakoshi, A [VerfasserIn]
Tateishi, K [VerfasserIn]
Tsuru, M [VerfasserIn]
Kono, A [VerfasserIn]

Themen:

106477-83-2
51110-01-1
8Y164V895Y
8ZYQ1474W7
Calcium
Carbachol
Chromogranin A
EC 2.4.2.31
EC 3.6.1.-
GTP-Binding Proteins
Journal Article
Pancreastatin
Pancreatic Hormones
Pertussis Toxin
SY7Q814VUP
Sodium Fluoride
Somatostatin
Virulence Factors, Bordetella

Anmerkungen:

Date Completed 12.06.1992

Date Revised 27.08.2019

published: Print

Citation Status MEDLINE

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM013314238