Expression of the cell surface antigen detected by the monoclonal antibody A7 in pancreatic carcinoma cell lines

In a previous study, we used a murine monoclonal antibody, A7, against human colon carcinoma as a drug-carrier to treat colorectal cancer. In the present study, we found that MAb A7 also reacted immunohistochemically with 73% of human pancreatic carcinoma cell lines, with the A7 antigen mainly being detected on the cell surface. However, the A7 antigen was found in only 9% of the spent media of these human pancreatic carcinoma cell lines by ELISA. On the other hand, the positive incidence of CA19-9, POA, ferritin, CEA, DU-PAN-2 and SLX in those spent media was 100%, 64%, 64%, 55%, 55% and 36%, respectively. These results suggest that the A7 antigen may only rarely be shed into the sera of pancreatic cancer patients, in which case MAb A7 could be a suitable drug-carrier in targeting chemotherapy for pancreatic cancer patients.

Medienart:

Artikel

Erscheinungsjahr:

1992

Erschienen:

1992

Enthalten in:

Zur Gesamtaufnahme - volume:22

Enthalten in:

Surgery today - 22(1992), 4 vom: 01., Seite 351-6

Sprache:

Englisch

Beteiligte Personen:

Otsuji, E [VerfasserIn]
Yamaguchi, T [VerfasserIn]
Yamaguchi, N [VerfasserIn]
Koyama, K [VerfasserIn]
Imanishi, J [VerfasserIn]
Yamaoka, N [VerfasserIn]
Takahashi, T [VerfasserIn]

Themen:

Antibodies, Monoclonal
Antigens, Neoplasm
Antigens, Surface
Journal Article
Research Support, Non-U.S. Gov't

Anmerkungen:

Date Completed 03.11.1992

Date Revised 18.09.2019

published: Print

Citation Status MEDLINE

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM013085506