Expression of the cell surface antigen detected by the monoclonal antibody A7 in pancreatic carcinoma cell lines
In a previous study, we used a murine monoclonal antibody, A7, against human colon carcinoma as a drug-carrier to treat colorectal cancer. In the present study, we found that MAb A7 also reacted immunohistochemically with 73% of human pancreatic carcinoma cell lines, with the A7 antigen mainly being detected on the cell surface. However, the A7 antigen was found in only 9% of the spent media of these human pancreatic carcinoma cell lines by ELISA. On the other hand, the positive incidence of CA19-9, POA, ferritin, CEA, DU-PAN-2 and SLX in those spent media was 100%, 64%, 64%, 55%, 55% and 36%, respectively. These results suggest that the A7 antigen may only rarely be shed into the sera of pancreatic cancer patients, in which case MAb A7 could be a suitable drug-carrier in targeting chemotherapy for pancreatic cancer patients.
Medienart: |
Artikel |
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Erscheinungsjahr: |
1992 |
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Erschienen: |
1992 |
Enthalten in: |
Zur Gesamtaufnahme - volume:22 |
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Enthalten in: |
Surgery today - 22(1992), 4 vom: 01., Seite 351-6 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Otsuji, E [VerfasserIn] |
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Themen: |
Antibodies, Monoclonal |
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Anmerkungen: |
Date Completed 03.11.1992 Date Revised 18.09.2019 published: Print Citation Status MEDLINE |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM013085506 |
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100 | 1 | |a Otsuji, E |e verfasserin |4 aut | |
245 | 1 | 0 | |a Expression of the cell surface antigen detected by the monoclonal antibody A7 in pancreatic carcinoma cell lines |
264 | 1 | |c 1992 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
338 | |a Band |b nc |2 rdacarrier | ||
500 | |a Date Completed 03.11.1992 | ||
500 | |a Date Revised 18.09.2019 | ||
500 | |a published: Print | ||
500 | |a Citation Status MEDLINE | ||
520 | |a In a previous study, we used a murine monoclonal antibody, A7, against human colon carcinoma as a drug-carrier to treat colorectal cancer. In the present study, we found that MAb A7 also reacted immunohistochemically with 73% of human pancreatic carcinoma cell lines, with the A7 antigen mainly being detected on the cell surface. However, the A7 antigen was found in only 9% of the spent media of these human pancreatic carcinoma cell lines by ELISA. On the other hand, the positive incidence of CA19-9, POA, ferritin, CEA, DU-PAN-2 and SLX in those spent media was 100%, 64%, 64%, 55%, 55% and 36%, respectively. These results suggest that the A7 antigen may only rarely be shed into the sera of pancreatic cancer patients, in which case MAb A7 could be a suitable drug-carrier in targeting chemotherapy for pancreatic cancer patients | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Research Support, Non-U.S. Gov't | |
650 | 7 | |a Antibodies, Monoclonal |2 NLM | |
650 | 7 | |a Antigens, Neoplasm |2 NLM | |
650 | 7 | |a Antigens, Surface |2 NLM | |
700 | 1 | |a Yamaguchi, T |e verfasserin |4 aut | |
700 | 1 | |a Yamaguchi, N |e verfasserin |4 aut | |
700 | 1 | |a Koyama, K |e verfasserin |4 aut | |
700 | 1 | |a Imanishi, J |e verfasserin |4 aut | |
700 | 1 | |a Yamaoka, N |e verfasserin |4 aut | |
700 | 1 | |a Takahashi, T |e verfasserin |4 aut | |
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