Identification and purification of the aspartate/glutamate carrier from bovine heart mitochondria
The aspartate/glutamate carrier from bovine heart mitochondria was solubilized with dodecyl-octaoxyethylene ether (C12E8) and purified by chromatography on hydroxyapatite and celite. On SDS gel electrophoresis, the purified aspartate/glutamate carrier consisted of a single protein band with an apparent Mr of 31,500. When reconstituted into liposomes the aspartate/glutamate carrier protein catalyzed an N-ethylmaleimide-sensitive aspartate/aspartate exchange. It was purified 620-fold with a recovery of 17.2% and a protein yield of 0.03% with respect to the mitochondrial extract. The properties of the reconstituted carrier, i.e. requirement for a counteranion, substrate specificity and inhibitor sensitivity, were similar to those of the aspartate/glutamate carrier as characterized in mitochondria.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
1992 |
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| Erschienen: |
1992 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:1106 |
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| Enthalten in: |
Biochimica et biophysica acta - 1106(1992), 2 vom: 21. Mai, Seite 291-6 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Bisaccia, F [VerfasserIn] |
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| Anmerkungen: |
Date Completed 08.07.1992 Date Revised 09.06.2019 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM012988502 |
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| 041 | |a eng | ||
| 100 | 1 | |a Bisaccia, F |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Identification and purification of the aspartate/glutamate carrier from bovine heart mitochondria |
| 264 | 1 | |c 1992 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 08.07.1992 | ||
| 500 | |a Date Revised 09.06.2019 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a The aspartate/glutamate carrier from bovine heart mitochondria was solubilized with dodecyl-octaoxyethylene ether (C12E8) and purified by chromatography on hydroxyapatite and celite. On SDS gel electrophoresis, the purified aspartate/glutamate carrier consisted of a single protein band with an apparent Mr of 31,500. When reconstituted into liposomes the aspartate/glutamate carrier protein catalyzed an N-ethylmaleimide-sensitive aspartate/aspartate exchange. It was purified 620-fold with a recovery of 17.2% and a protein yield of 0.03% with respect to the mitochondrial extract. The properties of the reconstituted carrier, i.e. requirement for a counteranion, substrate specificity and inhibitor sensitivity, were similar to those of the aspartate/glutamate carrier as characterized in mitochondria | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Amino Acid Transport Systems, Acidic |2 NLM | |
| 650 | 7 | |a Antiporters |2 NLM | |
| 650 | 7 | |a Carrier Proteins |2 NLM | |
| 650 | 7 | |a Detergents |2 NLM | |
| 650 | 7 | |a Glutamates |2 NLM | |
| 650 | 7 | |a Hydroxyapatites |2 NLM | |
| 650 | 7 | |a Liposomes |2 NLM | |
| 650 | 7 | |a Proteolipids |2 NLM | |
| 650 | 7 | |a aspartate-glutamate carrier |2 NLM | |
| 650 | 7 | |a proteoliposomes |2 NLM | |
| 650 | 7 | |a Aspartic Acid |2 NLM | |
| 650 | 7 | |a 30KYC7MIAI |2 NLM | |
| 650 | 7 | |a Durapatite |2 NLM | |
| 650 | 7 | |a 91D9GV0Z28 |2 NLM | |
| 700 | 1 | |a De Palma, A |e verfasserin |4 aut | |
| 700 | 1 | |a Palmieri, F |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Biochimica et biophysica acta |d 1948 |g 1106(1992), 2 vom: 21. Mai, Seite 291-6 |w (DE-627)NLM000000698 |x 0006-3002 |7 nnns |
| 773 | 1 | 8 | |g volume:1106 |g year:1992 |g number:2 |g day:21 |g month:05 |g pages:291-6 |
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