Tolerance induction in resting memory B cells specific for a protein antigen

Resting memory B lymphocytes specific for the model protein Ag cytochrome c have been shown to be susceptible to tolerance induction in in vitro splenic fragment cultures. This induction of nonresponsiveness is dependent upon the strength of the interaction between surface Ig and specific Ag, where concentration, valency, affinity, and time of exposure all appear to be important factors, as is the case for tolerance induction in immature or primary B cells. The induction of nonresponsivenes in greater than 80% of Ag-specific memory B cells was achieved by incubation with 1 microM cytochrome polymer for 24 h in the absence of T cell help. Not only were memory B cells unresponsive to specific Ag, they were also unable to become activated through nonspecific uptake and presentation of an Ag to which T cells have been primed, demonstrating that the induction of nonresponsiveness involves more than a modulation or blockade of surface Ig receptors. Although soluble factors collected from activated T cells failed to prevent memory B cells from becoming nonresponsive after surface Ig cross-linking, the direct activation of T cells within splenic fragment cultures did partially inhibit tolerance induction in splenic fragment memory B cells. In addition, the induction of tolerance was partially blocked by protein tyrosine kinase inhibitors, suggesting a physiologic change within the B cells associated with the state of nonresponsiveness and resulting from tyrosine-specific phosphorylation.

Medienart:

Artikel

Erscheinungsjahr:

1992

Erschienen:

1992

Enthalten in:

Zur Gesamtaufnahme - volume:148

Enthalten in:

Journal of immunology (Baltimore, Md. : 1950) - 148(1992), 9 vom: 01. Mai, Seite 2682-9

Sprache:

Englisch

Beteiligte Personen:

Johnson, J G [VerfasserIn]
Jemmerson, R [VerfasserIn]

Themen:

118409-60-2
1W306TDA6S
70563-58-5
Benzoquinones
Catechols
Cytochrome c Group
DH2M523P0H
EC 2.7.10.1
Genistein
Herbimycin
Immunoglobulins
Isoflavones
Journal Article
Lactams, Macrocyclic
Nitriles
Protein-Tyrosine Kinases
Quinones
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.
Rifabutin
Tyrphostin 47
Tyrphostins

Anmerkungen:

Date Completed 02.06.1992

Date Revised 03.12.2021

published: Print

Citation Status MEDLINE

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM012964379