Cloning and sequencing of human kappa-casein cDNA
A cDNA encoding kappa-casein of human milk was cloned and sequenced. The kappa-casein cDNA was isolated from a lambda gt11 library generated from mRNA prepared from a mammary gland biopsy obtained from a lactating woman. The library was screened with polyclonal rabbit antibodies raised against purified native kappa-casein. The obtained nucleotide sequence contained an ORF sufficient to encode the entire amino acid sequence of a kappa-casein precursor protein consisting of 182 amino acids. This includes a tentative signal peptide of 20 amino acids and a processed protein of 162 amino acids.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
1992 |
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| Erschienen: |
1992 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:3 |
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| Enthalten in: |
DNA sequence : the journal of DNA sequencing and mapping - 3(1992), 4 vom: 14., Seite 245-6 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Bergström, S [VerfasserIn] |
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| Themen: |
9007-49-2 |
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| Anmerkungen: |
Date Completed 27.04.1993 Date Revised 21.10.2019 published: Print GENBANK: X66417 Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM012777013 |
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| 008 | 231221s1992 xx ||||| 00| ||eng c | ||
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| 041 | |a eng | ||
| 100 | 1 | |a Bergström, S |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Cloning and sequencing of human kappa-casein cDNA |
| 264 | 1 | |c 1992 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 27.04.1993 | ||
| 500 | |a Date Revised 21.10.2019 | ||
| 500 | |a published: Print | ||
| 500 | |a GENBANK: X66417 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a A cDNA encoding kappa-casein of human milk was cloned and sequenced. The kappa-casein cDNA was isolated from a lambda gt11 library generated from mRNA prepared from a mammary gland biopsy obtained from a lactating woman. The library was screened with polyclonal rabbit antibodies raised against purified native kappa-casein. The obtained nucleotide sequence contained an ORF sufficient to encode the entire amino acid sequence of a kappa-casein precursor protein consisting of 182 amino acids. This includes a tentative signal peptide of 20 amino acids and a processed protein of 162 amino acids | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Caseins |2 NLM | |
| 650 | 7 | |a RNA, Messenger |2 NLM | |
| 650 | 7 | |a DNA |2 NLM | |
| 650 | 7 | |a 9007-49-2 |2 NLM | |
| 700 | 1 | |a Hansson, L |e verfasserin |4 aut | |
| 700 | 1 | |a Hernell, O |e verfasserin |4 aut | |
| 700 | 1 | |a Lönnerdal, B |e verfasserin |4 aut | |
| 700 | 1 | |a Nilsson, A K |e verfasserin |4 aut | |
| 700 | 1 | |a Strömqvist, M |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t DNA sequence : the journal of DNA sequencing and mapping |d 1990 |g 3(1992), 4 vom: 14., Seite 245-6 |w (DE-627)NLM012623776 |x 1042-5179 |7 nnns |
| 773 | 1 | 8 | |g volume:3 |g year:1992 |g number:4 |g day:14 |g pages:245-6 |
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| 952 | |d 3 |j 1992 |e 4 |b 14 |h 245-6 | ||