Gram scale purification and preparation of rabbit liver zinc metallothionein
A simplified procedure for purifying gram quantities of rabbit liver metallothionein (MT) using gel filtration and anion exchange chromatography is presented. The MT purification made use of anion exchange batch elution chromatography which greatly shortened the procedure. Quantitation techniques for use with crude and purified MT are discussed. This paper also describes the preparation of large amounts of ZnMT from Cd,ZnMT.
| Errataetall: | |
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| Medienart: |
Artikel |
| Erscheinungsjahr: |
1992 |
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| Erschienen: |
1992 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:22 |
|---|---|
| Enthalten in: |
Preparative biochemistry - 22(1992), 2 vom: 16. Juni, Seite 151-64 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Comeau, R D [VerfasserIn] |
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| Themen: |
9038-94-2 |
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| Anmerkungen: |
Date Completed 31.07.1992 Date Revised 28.10.2019 published: Print ErratumIn: Prep Biochem 1992 Sep-Dec;22(3-4):275 Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM012712639 |
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| 100 | 1 | |a Comeau, R D |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Gram scale purification and preparation of rabbit liver zinc metallothionein |
| 264 | 1 | |c 1992 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 31.07.1992 | ||
| 500 | |a Date Revised 28.10.2019 | ||
| 500 | |a published: Print | ||
| 500 | |a ErratumIn: Prep Biochem 1992 Sep-Dec;22(3-4):275 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a A simplified procedure for purifying gram quantities of rabbit liver metallothionein (MT) using gel filtration and anion exchange chromatography is presented. The MT purification made use of anion exchange batch elution chromatography which greatly shortened the procedure. Quantitation techniques for use with crude and purified MT are discussed. This paper also describes the preparation of large amounts of ZnMT from Cd,ZnMT | ||
| 650 | 4 | |a Journal Article | |
| 650 | 7 | |a zinc thionein |2 NLM | |
| 650 | 7 | |a Metallothionein |2 NLM | |
| 650 | 7 | |a 9038-94-2 |2 NLM | |
| 650 | 7 | |a Zinc |2 NLM | |
| 650 | 7 | |a J41CSQ7QDS |2 NLM | |
| 700 | 1 | |a McDonald, K W |e verfasserin |4 aut | |
| 700 | 1 | |a Tolman, G L |e verfasserin |4 aut | |
| 700 | 1 | |a Vasak, M |e verfasserin |4 aut | |
| 700 | 1 | |a Liberatore, F A |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Preparative biochemistry |d 1971 |g 22(1992), 2 vom: 16. Juni, Seite 151-64 |w (DE-627)NLM000034630 |x 0032-7484 |7 nnns |
| 773 | 1 | 8 | |g volume:22 |g year:1992 |g number:2 |g day:16 |g month:06 |g pages:151-64 |
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