Clinical applications of radioimmunoassay of the alpha-subunit of glycoprotein hormones
The radioimmunoassay of alpha-subunit adapted in our laboratory was widely evaluated. Three different antisera (anti-pituitary alpha-subunit, anti-alpha-TSH and anti-alpha-hCG), the labelled preparations of pituitary alpha-subunit and alpha-hCG, and cross-reactivity with intact glycoprotein hormones (MRC standards of LH, FSH, hCG and TSH) were tested for their potential influence on the results of the assay. The basal levels of alpha-subunit were measured in 48 healthy young men, 48 normally menstruating women, 33 menopausal women, 37 pregnant women and 70 patients with pituitary adenoma. In addition a possibility of pulsatile secretion of alpha-subunit was investigated in 9 healthy young women, the ranges of alpha-subunit concentrations found were as follow (means +/- SD): 1.2 +/- 0.4 micrograms/l--in young men, 1.1 +/- 0.4 micrograms/l--in young women, 3.2 +/- 0.7 micrograms/l--in postmenopausal women, 1-54 micrograms/l--in pregnant women, and between 2.6 and 44.0 micrograms/l in 14 of 70 patients with pituitary adenoma. There were good correlations of results for 3 different antisera and their cross-reactivity with LH, FSH, hCG and TSH were just as low. In conclusion, the alpha-subunit assay appears clinically useful and should be widely applied in routine endocrinological diagnostics.
Medienart: |
Artikel |
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Erscheinungsjahr: |
1992 |
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Erschienen: |
1992 |
Enthalten in: |
Zur Gesamtaufnahme - volume:43 |
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Enthalten in: |
Endokrynologia Polska - 43(1992), 3 vom: 28., Seite 250-4 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Jeske, W [VerfasserIn] |
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Themen: |
Glycoprotein Hormones, alpha Subunit |
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Anmerkungen: |
Date Completed 13.09.1994 Date Revised 02.03.2017 published: Print Citation Status MEDLINE |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM012660361 |
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245 | 1 | 0 | |a Clinical applications of radioimmunoassay of the alpha-subunit of glycoprotein hormones |
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500 | |a Date Completed 13.09.1994 | ||
500 | |a Date Revised 02.03.2017 | ||
500 | |a published: Print | ||
500 | |a Citation Status MEDLINE | ||
520 | |a The radioimmunoassay of alpha-subunit adapted in our laboratory was widely evaluated. Three different antisera (anti-pituitary alpha-subunit, anti-alpha-TSH and anti-alpha-hCG), the labelled preparations of pituitary alpha-subunit and alpha-hCG, and cross-reactivity with intact glycoprotein hormones (MRC standards of LH, FSH, hCG and TSH) were tested for their potential influence on the results of the assay. The basal levels of alpha-subunit were measured in 48 healthy young men, 48 normally menstruating women, 33 menopausal women, 37 pregnant women and 70 patients with pituitary adenoma. In addition a possibility of pulsatile secretion of alpha-subunit was investigated in 9 healthy young women, the ranges of alpha-subunit concentrations found were as follow (means +/- SD): 1.2 +/- 0.4 micrograms/l--in young men, 1.1 +/- 0.4 micrograms/l--in young women, 3.2 +/- 0.7 micrograms/l--in postmenopausal women, 1-54 micrograms/l--in pregnant women, and between 2.6 and 44.0 micrograms/l in 14 of 70 patients with pituitary adenoma. There were good correlations of results for 3 different antisera and their cross-reactivity with LH, FSH, hCG and TSH were just as low. In conclusion, the alpha-subunit assay appears clinically useful and should be widely applied in routine endocrinological diagnostics | ||
650 | 4 | |a Journal Article | |
650 | 7 | |a Glycoprotein Hormones, alpha Subunit |2 NLM | |
650 | 7 | |a Immune Sera |2 NLM | |
700 | 1 | |a Zgliczyński, W |e verfasserin |4 aut | |
700 | 1 | |a Zgliczyński, S |e verfasserin |4 aut | |
700 | 1 | |a Kasperlik-Załuska, A |e verfasserin |4 aut | |
700 | 1 | |a Stopińska, U |e verfasserin |4 aut | |
700 | 1 | |a Soszyński, P |e verfasserin |4 aut | |
700 | 1 | |a Zieliński, M |e verfasserin |4 aut | |
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