Photosensitivity of 8BrcGMP-induced conductance in ROS-excised patches
Inside-out patches from ROS plasma membranes contain the basic enzymes of the phototransduction cascade. Similar to a native photoreceptor cell, such patches are capable of responding to light, the effect of which suppresses the cGMP-activated current. Photoresponses are observed only in the presence of GTP, whereas ATP essentially accelerates the current recovery to a dark level. Photoresponses are also observed in the presence of 8BrcGMP. Phosphodiesterase (PDE) hydrolyzes 8BrcGMP two orders of magnitude slower than cGMP, so the light inhibition of the 8BrcGMP-induced current cannot be accounted for by PDE activation. It seems that activity of cGMP-gated channels depends not only on cGMP concentration, but is additionally controlled by some other regulatory mechanisms.
Medienart: |
Artikel |
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Erscheinungsjahr: |
1992 |
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Erschienen: |
1992 |
Enthalten in: |
Zur Gesamtaufnahme - volume:305 |
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Enthalten in: |
FEBS letters - 305(1992), 3 vom: 06. Juli, Seite 174-6 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Kosolapov, A V [VerfasserIn] |
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Themen: |
1-Methyl-3-isobutylxanthine |
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Anmerkungen: |
Date Completed 19.05.1993 Date Revised 20.06.2019 published: Print Citation Status MEDLINE |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM012649554 |
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245 | 1 | 0 | |a Photosensitivity of 8BrcGMP-induced conductance in ROS-excised patches |
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500 | |a Date Completed 19.05.1993 | ||
500 | |a Date Revised 20.06.2019 | ||
500 | |a published: Print | ||
500 | |a Citation Status MEDLINE | ||
520 | |a Inside-out patches from ROS plasma membranes contain the basic enzymes of the phototransduction cascade. Similar to a native photoreceptor cell, such patches are capable of responding to light, the effect of which suppresses the cGMP-activated current. Photoresponses are observed only in the presence of GTP, whereas ATP essentially accelerates the current recovery to a dark level. Photoresponses are also observed in the presence of 8BrcGMP. Phosphodiesterase (PDE) hydrolyzes 8BrcGMP two orders of magnitude slower than cGMP, so the light inhibition of the 8BrcGMP-induced current cannot be accounted for by PDE activation. It seems that activity of cGMP-gated channels depends not only on cGMP concentration, but is additionally controlled by some other regulatory mechanisms | ||
650 | 4 | |a Journal Article | |
650 | 7 | |a 8-bromocyclic GMP |2 NLM | |
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