The immunodiagnosis of thrombocyte membrane glycoprotein deficiencies : Glanzmann's thrombasthenia, Bernard-Soulier syndrome and GMP-140 protein deficiency
Immunological methods were developed for the diagnosis of platelet membrane glycoprotein (GP) deficiencies. The number of membrane GP on platelet surface was determined as the binding of 125I-labeled monoclonal antibodies (mAB) directed against individual platelet GP. Total amount of GP in platelet lysate was assessed by immunoblotting with specific polyclonal antibodies. Methods were applied for patients with different thrombocytopathies. Binding of mAB VM16a, directed against GP IIb-IIIa was strongly decreased in patients with Glanzmann's thrombasthenia (0.5-14.5% of normal) and binding of anti-GP Ib mAB VM16d--in patient with Bernard-Soulier syndrome (0.5% of control) indicating the deficiencies of corresponding GP. In patient with gray platelet syndrome binding of both antibodies was not decreased but even increased. It was shown by immunoblotting that platelets from the patient with gray platelet syndrome contained normal amount of GP IIa, but strongly decreased amount of GMP-140 (14.5% of control)--membrane GP of platelet--granules.
| Medienart: |
Artikel |
|---|
| Erscheinungsjahr: |
1992 |
|---|---|
| Erschienen: |
1992 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:114 |
|---|---|
| Enthalten in: |
Biulleten' eksperimental'noi biologii i meditsiny - 114(1992), 12 vom: 25. Dez., Seite 635-7 |
| Sprache: |
Russisch |
|---|
| Weiterer Titel: |
Immunodiagnostika defitsitov membrannykh glikoproteinov trombotsitov: trombastenii Glantsmanna, sindroma Bernara--Sul'e i defitsita belka GMP-140 |
|---|
| Beteiligte Personen: |
Agafonova, O G [VerfasserIn] |
|---|
| Themen: |
Antibodies, Monoclonal |
|---|
| Anmerkungen: |
Date Completed 02.04.1993 Date Revised 19.11.2015 published: Print Citation Status MEDLINE |
|---|
| Förderinstitution / Projekttitel: |
|
|---|
| PPN (Katalog-ID): |
NLM012645648 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM012645648 | ||
| 003 | DE-627 | ||
| 005 | 20231221053159.0 | ||
| 007 | tu | ||
| 008 | 231221s1992 xx ||||| 00| ||rus c | ||
| 028 | 5 | 2 | |a pubmed24n0043.xml |
| 035 | |a (DE-627)NLM012645648 | ||
| 035 | |a (NLM)1284039 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a rus | ||
| 100 | 1 | |a Agafonova, O G |e verfasserin |4 aut | |
| 245 | 1 | 4 | |a The immunodiagnosis of thrombocyte membrane glycoprotein deficiencies |b Glanzmann's thrombasthenia, Bernard-Soulier syndrome and GMP-140 protein deficiency |
| 246 | 3 | 3 | |a Immunodiagnostika defitsitov membrannykh glikoproteinov trombotsitov: trombastenii Glantsmanna, sindroma Bernara--Sul'e i defitsita belka GMP-140 |
| 264 | 1 | |c 1992 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 02.04.1993 | ||
| 500 | |a Date Revised 19.11.2015 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Immunological methods were developed for the diagnosis of platelet membrane glycoprotein (GP) deficiencies. The number of membrane GP on platelet surface was determined as the binding of 125I-labeled monoclonal antibodies (mAB) directed against individual platelet GP. Total amount of GP in platelet lysate was assessed by immunoblotting with specific polyclonal antibodies. Methods were applied for patients with different thrombocytopathies. Binding of mAB VM16a, directed against GP IIb-IIIa was strongly decreased in patients with Glanzmann's thrombasthenia (0.5-14.5% of normal) and binding of anti-GP Ib mAB VM16d--in patient with Bernard-Soulier syndrome (0.5% of control) indicating the deficiencies of corresponding GP. In patient with gray platelet syndrome binding of both antibodies was not decreased but even increased. It was shown by immunoblotting that platelets from the patient with gray platelet syndrome contained normal amount of GP IIa, but strongly decreased amount of GMP-140 (14.5% of control)--membrane GP of platelet--granules | ||
| 650 | 4 | |a Comparative Study | |
| 650 | 4 | |a English Abstract | |
| 650 | 4 | |a Journal Article | |
| 650 | 7 | |a Antibodies, Monoclonal |2 NLM | |
| 650 | 7 | |a P-Selectin |2 NLM | |
| 650 | 7 | |a Platelet Membrane Glycoproteins |2 NLM | |
| 700 | 1 | |a Vinogradov, D V |e verfasserin |4 aut | |
| 700 | 1 | |a Khaspekova, S G |e verfasserin |4 aut | |
| 700 | 1 | |a Lagutina, N Ia |e verfasserin |4 aut | |
| 700 | 1 | |a Labinskaia, T A |e verfasserin |4 aut | |
| 700 | 1 | |a Vasil'ev, S A |e verfasserin |4 aut | |
| 700 | 1 | |a Mazurov, A V |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Biulleten' eksperimental'noi biologii i meditsiny |d 1945 |g 114(1992), 12 vom: 25. Dez., Seite 635-7 |w (DE-627)NLM00000118X |x 0365-9615 |7 nnns |
| 773 | 1 | 8 | |g volume:114 |g year:1992 |g number:12 |g day:25 |g month:12 |g pages:635-7 |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 114 |j 1992 |e 12 |b 25 |c 12 |h 635-7 | ||