Both open reading frames of the linear plasmid pMC3-2 from the ascomycete Morchella conica are transcribed in vivo
Mitochondrial RNA was isolated from the morel strain Morchella conica 3 harbouring the linear plasmid pMC3-2 and subjected to gel electrophoresis followed by a Northern analysis using cloned fragments of the plasmid pMC3-2 as probes. Hybridization was obtained only with central parts of pMC3-2 and specific bands of mtRNA. The hybridization bands (2.8 kb and 1.0 kb) correspond in size to the length of the two ORFs of pMC3-2 which were deduced from nucleotide-sequence data. Thus, both ORFs, one encoding a DNA polymerase and the other a yet unknown protein, are transcribed in the mitochondria of the plasmid-bearing Morchella conica strain.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
1992 |
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| Erschienen: |
1992 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:22 |
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| Enthalten in: |
Current genetics - 22(1992), 6 vom: 24. Dez., Seite 507-9 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Rohe, M [VerfasserIn] |
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| Themen: |
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| Anmerkungen: |
Date Completed 02.02.1993 Date Revised 07.09.2019 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM012624349 |
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| 100 | 1 | |a Rohe, M |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Both open reading frames of the linear plasmid pMC3-2 from the ascomycete Morchella conica are transcribed in vivo |
| 264 | 1 | |c 1992 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 02.02.1993 | ||
| 500 | |a Date Revised 07.09.2019 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Mitochondrial RNA was isolated from the morel strain Morchella conica 3 harbouring the linear plasmid pMC3-2 and subjected to gel electrophoresis followed by a Northern analysis using cloned fragments of the plasmid pMC3-2 as probes. Hybridization was obtained only with central parts of pMC3-2 and specific bands of mtRNA. The hybridization bands (2.8 kb and 1.0 kb) correspond in size to the length of the two ORFs of pMC3-2 which were deduced from nucleotide-sequence data. Thus, both ORFs, one encoding a DNA polymerase and the other a yet unknown protein, are transcribed in the mitochondria of the plasmid-bearing Morchella conica strain | ||
| 650 | 4 | |a Journal Article | |
| 650 | 7 | |a RNA, Fungal |2 NLM | |
| 650 | 7 | |a RNA, Mitochondrial |2 NLM | |
| 650 | 7 | |a RNA |2 NLM | |
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| 700 | 1 | |a Meinhardt, F |e verfasserin |4 aut | |
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