Anticodon-dependent aminoacylation of RNA minisubstrate by lysyl-tRNA synthetase
Specific inhibition of mammalian lysyl-tRNA synthetase by polyU is shown. Inhibition of the enzyme is dependent on the length of the oligonucleotide, since oligoU molecules with a length of less than 8 residues do not inhibit the aminoacylation, whilst the effect of oligoU molecules with a length of about 30 residues is the same as that of polyU. Inhibition is a result of recognition by the enzyme of the tRNALys anticodon sequence (UUU) coded by polyU. Aminoacylation of the oligoU molecule with attached CCA sequence (G(U)20-CCA) by yeast and mammalian lysyl-tRNA synthetases is demonstrated.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
1992 |
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| Erschienen: |
1992 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:314 |
|---|---|
| Enthalten in: |
FEBS letters - 314(1992), 3 vom: 21. Dez., Seite 256-8 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Khvorova, A M [VerfasserIn] |
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| Themen: |
27416-86-0 |
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| Anmerkungen: |
Date Completed 25.01.1993 Date Revised 20.06.2019 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM01262098X |
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| 100 | 1 | |a Khvorova, A M |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Anticodon-dependent aminoacylation of RNA minisubstrate by lysyl-tRNA synthetase |
| 264 | 1 | |c 1992 | |
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| 500 | |a Date Completed 25.01.1993 | ||
| 500 | |a Date Revised 20.06.2019 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Specific inhibition of mammalian lysyl-tRNA synthetase by polyU is shown. Inhibition of the enzyme is dependent on the length of the oligonucleotide, since oligoU molecules with a length of less than 8 residues do not inhibit the aminoacylation, whilst the effect of oligoU molecules with a length of about 30 residues is the same as that of polyU. Inhibition is a result of recognition by the enzyme of the tRNALys anticodon sequence (UUU) coded by polyU. Aminoacylation of the oligoU molecule with attached CCA sequence (G(U)20-CCA) by yeast and mammalian lysyl-tRNA synthetases is demonstrated | ||
| 650 | 4 | |a Journal Article | |
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| 700 | 1 | |a Gladilin, K L |e verfasserin |4 aut | |
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