Human monoclonal antibodies against Plasmodium falciparum : production, stabilization and characterization
Nine human monoclonal antibodies (MoAbs) recognizing 7 different antigenic structures of blood-stages of the human malarial parasite P. falciparum (Pf) were produced by Epstein-Barr virus transformed B-cell lines (EBV-TCL) with or without fusion to the lymphoblastoid cell line KR4. The peripheral blood B-lymphocytes were obtained from 8 Gambian donors immune to Pf malaria. Two of the EBV-TCL could be expanded and maintained for more than 6 months but neither one could be cloned. Six additional EBV-TCL were stabilized after fusion with the KR4 lymphoblastoid cell line. All resulting hybridomas permitted easy cloning. Some of the MoAbs produced distinct fluorescent staining patterns of asexual Pf blood-stage parasites when using high-resolution digitized video-intensified fluorescence microscopy. Antigens on 195 kD and 155 kD proteins were recognized by 3 and 1 MoAb, respectively, using Western blotting and immunoprecipitation techniques.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
1992 |
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| Erschienen: |
1992 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:14 |
|---|---|
| Enthalten in: |
Parasite immunology - 14(1992), 4 vom: 22. Juli, Seite 451-6 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Kamber, M [VerfasserIn] |
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| Themen: |
Antibodies, Monoclonal |
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| Anmerkungen: |
Date Completed 03.12.1992 Date Revised 11.09.2019 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM012595756 |
|---|
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|---|---|---|---|
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| 008 | 231221s1992 xx ||||| 00| ||eng c | ||
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| 035 | |a (DE-627)NLM012595756 | ||
| 035 | |a (NLM)1279503 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Kamber, M |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Human monoclonal antibodies against Plasmodium falciparum |b production, stabilization and characterization |
| 264 | 1 | |c 1992 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 03.12.1992 | ||
| 500 | |a Date Revised 11.09.2019 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Nine human monoclonal antibodies (MoAbs) recognizing 7 different antigenic structures of blood-stages of the human malarial parasite P. falciparum (Pf) were produced by Epstein-Barr virus transformed B-cell lines (EBV-TCL) with or without fusion to the lymphoblastoid cell line KR4. The peripheral blood B-lymphocytes were obtained from 8 Gambian donors immune to Pf malaria. Two of the EBV-TCL could be expanded and maintained for more than 6 months but neither one could be cloned. Six additional EBV-TCL were stabilized after fusion with the KR4 lymphoblastoid cell line. All resulting hybridomas permitted easy cloning. Some of the MoAbs produced distinct fluorescent staining patterns of asexual Pf blood-stage parasites when using high-resolution digitized video-intensified fluorescence microscopy. Antigens on 195 kD and 155 kD proteins were recognized by 3 and 1 MoAb, respectively, using Western blotting and immunoprecipitation techniques | ||
| 650 | 4 | |a Journal Article | |
| 650 | 7 | |a Antibodies, Monoclonal |2 NLM | |
| 650 | 7 | |a Antibodies, Protozoan |2 NLM | |
| 650 | 7 | |a Antigens, Protozoan |2 NLM | |
| 650 | 7 | |a Epitopes |2 NLM | |
| 700 | 1 | |a Blackman, M |e verfasserin |4 aut | |
| 700 | 1 | |a Lin, P S |e verfasserin |4 aut | |
| 700 | 1 | |a Brown, J |e verfasserin |4 aut | |
| 700 | 1 | |a Whittle, H |e verfasserin |4 aut | |
| 700 | 1 | |a Schmidt-Ullrich, R |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Parasite immunology |d 1982 |g 14(1992), 4 vom: 22. Juli, Seite 451-6 |w (DE-627)NLM000464953 |x 1365-3024 |7 nnns |
| 773 | 1 | 8 | |g volume:14 |g year:1992 |g number:4 |g day:22 |g month:07 |g pages:451-6 |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 14 |j 1992 |e 4 |b 22 |c 07 |h 451-6 | ||