Guanylate cyclase in Escherichia coli. I. Purification of the enzyme and evidence for an inhibitor
Guanylate cyclase activity is present in crude E. coli extract. Guanylate cyclase has been purified 3500 fold from this extract, through ammonium sulfate fractionation, DEAE-cellulose chromatography. Sephadex G-75 gel-filtration and polyacrylamide gel preparative microelectrophoresis. During the purification a guanylate cyclase inhibitor has been separated.
| Medienart: |
Artikel |
|---|
| Erscheinungsjahr: |
1978 |
|---|---|
| Erschienen: |
1978 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:172 |
|---|---|
| Enthalten in: |
Comptes rendus des seances de la Societe de biologie et de ses filiales - 172(1978), 5 vom: 24., Seite 860-7 |
| Sprache: |
Französisch |
|---|
| Weiterer Titel: |
Guanylate cyclase chez Escherichia coli. I. Purification de l'enzyme et mise en évidence d'un inhibiteur |
|---|
| Beteiligte Personen: |
Macchia, V [VerfasserIn] |
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| Themen: |
Bacterial Proteins |
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| Anmerkungen: |
Date Completed 17.10.1979 Date Revised 09.12.2020 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM000391891 |
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| 245 | 1 | 0 | |a Guanylate cyclase in Escherichia coli. I. Purification of the enzyme and evidence for an inhibitor |
| 246 | 3 | 3 | |a Guanylate cyclase chez Escherichia coli. I. Purification de l'enzyme et mise en évidence d'un inhibiteur |
| 264 | 1 | |c 1978 | |
| 336 | |a Text |b txt |2 rdacontent | ||
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| 500 | |a Date Completed 17.10.1979 | ||
| 500 | |a Date Revised 09.12.2020 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Guanylate cyclase activity is present in crude E. coli extract. Guanylate cyclase has been purified 3500 fold from this extract, through ammonium sulfate fractionation, DEAE-cellulose chromatography. Sephadex G-75 gel-filtration and polyacrylamide gel preparative microelectrophoresis. During the purification a guanylate cyclase inhibitor has been separated | ||
| 650 | 4 | |a Journal Article | |
| 650 | 7 | |a Bacterial Proteins |2 NLM | |
| 650 | 7 | |a Enzyme Inhibitors |2 NLM | |
| 650 | 7 | |a Guanylate Cyclase |2 NLM | |
| 650 | 7 | |a EC 4.6.1.2 |2 NLM | |
| 700 | 1 | |a Rocino, A |e verfasserin |4 aut | |
| 700 | 1 | |a Gulletta, E |e verfasserin |4 aut | |
| 700 | 1 | |a Consiglio, E |e verfasserin |4 aut | |
| 700 | 1 | |a Varrone, S |e verfasserin |4 aut | |
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