Expression of differentiated functions in hepatoma cell hybrids : IX extinction and reexpression of liver-specific enzymes in rat hepatoma-Chinese hamster fibroblast hybrids
Most of the hybrid clones derived from a cross of Chinese hamster fibroblasts (DON) with rat hepatoma cells (Faza 967) showed preferential loss of rat chromosomes. Two of the hybrid clones retained the rat chromosomes, and both showed extinction of 4 liver-specific enzymes: aldolase B, liver alcohol dehydrogenase, and the inducible enzymes tyrosine aminotransferase and alanine aminotransferase. Subcloning of 1 of these hybrids, which contained 2 sets of hepatoma chromosomes and 1 set of hamster chromosomes, permitted the isolation of some clones which reexpressed 1 or more of the liver-specific enzymes. Liver alcohol dehydrogenase was the most frequently reexpressed enzyme and aldolase B the least. Tyrosine aminotransferase inducibility was reexpressed independently of basal activity, and the enzyme produced by the reexpressing hybrid cells was precipitated by a specific antiserum. No correlation was detected between the presence or absence of the marker chromosomes (large metacentrics) of the hamster parent and the extinction and reexpression of the hepatic enzymes. The results reported confirm and extend to interspecific hybrids the observation of the stable and independent reexpression of tissue-specific enzymes.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
1975 |
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| Erschienen: |
1975 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:1 |
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| Enthalten in: |
Somatic cell genetics - 1(1975), 1 vom: 09. Jan., Seite 27-40 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Weiss, M C [VerfasserIn] |
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| Themen: |
Alanine Transaminase |
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| Anmerkungen: |
Date Completed 29.07.1977 Date Revised 27.10.2019 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM00018005X |
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| 041 | |a eng | ||
| 100 | 1 | |a Weiss, M C |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Expression of differentiated functions in hepatoma cell hybrids |b IX extinction and reexpression of liver-specific enzymes in rat hepatoma-Chinese hamster fibroblast hybrids |
| 264 | 1 | |c 1975 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 29.07.1977 | ||
| 500 | |a Date Revised 27.10.2019 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Most of the hybrid clones derived from a cross of Chinese hamster fibroblasts (DON) with rat hepatoma cells (Faza 967) showed preferential loss of rat chromosomes. Two of the hybrid clones retained the rat chromosomes, and both showed extinction of 4 liver-specific enzymes: aldolase B, liver alcohol dehydrogenase, and the inducible enzymes tyrosine aminotransferase and alanine aminotransferase. Subcloning of 1 of these hybrids, which contained 2 sets of hepatoma chromosomes and 1 set of hamster chromosomes, permitted the isolation of some clones which reexpressed 1 or more of the liver-specific enzymes. Liver alcohol dehydrogenase was the most frequently reexpressed enzyme and aldolase B the least. Tyrosine aminotransferase inducibility was reexpressed independently of basal activity, and the enzyme produced by the reexpressing hybrid cells was precipitated by a specific antiserum. No correlation was detected between the presence or absence of the marker chromosomes (large metacentrics) of the hamster parent and the extinction and reexpression of the hepatic enzymes. The results reported confirm and extend to interspecific hybrids the observation of the stable and independent reexpression of tissue-specific enzymes | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, U.S. Gov't, P.H.S. | |
| 650 | 7 | |a Alcohol Oxidoreductases |2 NLM | |
| 650 | 7 | |a EC 1.1.- |2 NLM | |
| 650 | 7 | |a Alanine Transaminase |2 NLM | |
| 650 | 7 | |a EC 2.6.1.2 |2 NLM | |
| 650 | 7 | |a Tyrosine Transaminase |2 NLM | |
| 650 | 7 | |a EC 2.6.1.5 |2 NLM | |
| 650 | 7 | |a Fructose-Bisphosphate Aldolase |2 NLM | |
| 650 | 7 | |a EC 4.1.2.13 |2 NLM | |
| 700 | 1 | |a Sparkes, R S |e verfasserin |4 aut | |
| 700 | 1 | |a Bertolotti, R |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Somatic cell genetics |d 1982 |g 1(1975), 1 vom: 09. Jan., Seite 27-40 |w (DE-627)NLM000180041 |x 0098-0366 |7 nnns |
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| 952 | |d 1 |j 1975 |e 1 |b 09 |c 01 |h 27-40 | ||