Identification of the 30 S protein adjacent to peptidyl transferase catalytic center of Escherichia coli ribosomes

Iodoacetylphenylalanyl-tRNAPhe was used as an affinity label to localize the ribosomal components involved in the peptidyl transferase catalytic center of Escherichia coli ribosomes. When labeling was carried out at pH 5.0, the affinity label could specifically label the ribosomal components which comprise the catalytic center. Analysis of ribosomal proteins which had reacted with the affinity label revealed that a 30 S subunit protein, S 20, was located at or near to the ribosomal binding site of the 3'-terminus of aminoacyl- or peptidyl-tRNA.

Medienart:

Artikel

Erscheinungsjahr:

1975

Erschienen:

1975

Enthalten in:

Zur Gesamtaufnahme - volume:2

Enthalten in:

Molecular biology reports - 2(1975), 3 vom: 31. Okt., Seite 282-8

Sprache:

Englisch

Beteiligte Personen:

Hatayama, T [VerfasserIn]
Yukioka, M [VerfasserIn]
Morisawa, S [VerfasserIn]

Themen:

47E5O17Y3R
9014-25-9
Acyltransferases
EC 2.3.-
EC 2.3.2.12
Iodoacetates
Journal Article
Macromolecular Substances
Peptidyl Transferases
Phenylalanine
RNA, Transfer
Ribosomal Proteins

Anmerkungen:

Date Completed 19.02.1976

Date Revised 28.10.2019

published: Print

Citation Status MEDLINE

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM000008176