Identification of the 30 S protein adjacent to peptidyl transferase catalytic center of Escherichia coli ribosomes
Iodoacetylphenylalanyl-tRNAPhe was used as an affinity label to localize the ribosomal components involved in the peptidyl transferase catalytic center of Escherichia coli ribosomes. When labeling was carried out at pH 5.0, the affinity label could specifically label the ribosomal components which comprise the catalytic center. Analysis of ribosomal proteins which had reacted with the affinity label revealed that a 30 S subunit protein, S 20, was located at or near to the ribosomal binding site of the 3'-terminus of aminoacyl- or peptidyl-tRNA.
Medienart: |
Artikel |
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Erscheinungsjahr: |
1975 |
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Erschienen: |
1975 |
Enthalten in: |
Zur Gesamtaufnahme - volume:2 |
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Enthalten in: |
Molecular biology reports - 2(1975), 3 vom: 31. Okt., Seite 282-8 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Hatayama, T [VerfasserIn] |
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Themen: |
47E5O17Y3R |
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Anmerkungen: |
Date Completed 19.02.1976 Date Revised 28.10.2019 published: Print Citation Status MEDLINE |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM000008176 |
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100 | 1 | |a Hatayama, T |e verfasserin |4 aut | |
245 | 1 | 0 | |a Identification of the 30 S protein adjacent to peptidyl transferase catalytic center of Escherichia coli ribosomes |
264 | 1 | |c 1975 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
338 | |a Band |b nc |2 rdacarrier | ||
500 | |a Date Completed 19.02.1976 | ||
500 | |a Date Revised 28.10.2019 | ||
500 | |a published: Print | ||
500 | |a Citation Status MEDLINE | ||
520 | |a Iodoacetylphenylalanyl-tRNAPhe was used as an affinity label to localize the ribosomal components involved in the peptidyl transferase catalytic center of Escherichia coli ribosomes. When labeling was carried out at pH 5.0, the affinity label could specifically label the ribosomal components which comprise the catalytic center. Analysis of ribosomal proteins which had reacted with the affinity label revealed that a 30 S subunit protein, S 20, was located at or near to the ribosomal binding site of the 3'-terminus of aminoacyl- or peptidyl-tRNA | ||
650 | 4 | |a Journal Article | |
650 | 7 | |a Iodoacetates |2 NLM | |
650 | 7 | |a Macromolecular Substances |2 NLM | |
650 | 7 | |a Ribosomal Proteins |2 NLM | |
650 | 7 | |a Phenylalanine |2 NLM | |
650 | 7 | |a 47E5O17Y3R |2 NLM | |
650 | 7 | |a RNA, Transfer |2 NLM | |
650 | 7 | |a 9014-25-9 |2 NLM | |
650 | 7 | |a Acyltransferases |2 NLM | |
650 | 7 | |a EC 2.3.- |2 NLM | |
650 | 7 | |a Peptidyl Transferases |2 NLM | |
650 | 7 | |a EC 2.3.2.12 |2 NLM | |
700 | 1 | |a Yukioka, M |e verfasserin |4 aut | |
700 | 1 | |a Morisawa, S |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Molecular biology reports |d 1973 |g 2(1975), 3 vom: 31. Okt., Seite 282-8 |w (DE-627)NLM000008168 |x 1573-4978 |7 nnns |
773 | 1 | 8 | |g volume:2 |g year:1975 |g number:3 |g day:31 |g month:10 |g pages:282-8 |
912 | |a GBV_USEFLAG_A | ||
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952 | |d 2 |j 1975 |e 3 |b 31 |c 10 |h 282-8 |