Very High Throughput Electrical Cell Lysis and Extraction of Intracellular Compounds Using 3D Carbon Electrodes in Lab-on-a-Chip Devices

Here we present an electrical lysis throughput of 600 microliters per minute at high cell density (10<sup>8</sup> yeast cells per ml) with 90% efficiency, thus improving the current common throughput of one microliter per minute. We also demonstrate the extraction of intracellular luciferase from mammalian cells with efficiency comparable to off-chip bulk chemical lysis. The goal of this work is to develop a sample preparation module that can act as a stand-alone device or be integrated to other functions already demonstrated in miniaturized devices, including sorting and analysis, towards a true lab-on-a-chip..

Medienart:

E-Artikel

Erscheinungsjahr:

2012

Erschienen:

2012

Enthalten in:

Zur Gesamtaufnahme - volume:3

Enthalten in:

Micromachines - 3(2012), 3, Seite 574-581

Sprache:

Englisch

Beteiligte Personen:

Philippe Renaud [VerfasserIn]
Freddy Radtke [VerfasserIn]
Rajwinder Lehal [VerfasserIn]
Rodrigo Martinez-Duarte [VerfasserIn]
Guillaume Mernier [VerfasserIn]

Links:

doi.org [kostenfrei]
doaj.org [kostenfrei]
www.mdpi.com [kostenfrei]
Journal toc [kostenfrei]

Themen:

Carbon electrodes
Cell lysis
Mechanical engineering and machinery
Microfluidics

doi:

10.3390/mi3030574

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

DOAJ060954248