Very High Throughput Electrical Cell Lysis and Extraction of Intracellular Compounds Using 3D Carbon Electrodes in Lab-on-a-Chip Devices
Here we present an electrical lysis throughput of 600 microliters per minute at high cell density (10<sup>8</sup> yeast cells per ml) with 90% efficiency, thus improving the current common throughput of one microliter per minute. We also demonstrate the extraction of intracellular luciferase from mammalian cells with efficiency comparable to off-chip bulk chemical lysis. The goal of this work is to develop a sample preparation module that can act as a stand-alone device or be integrated to other functions already demonstrated in miniaturized devices, including sorting and analysis, towards a true lab-on-a-chip..
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2012 |
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Erschienen: |
2012 |
Enthalten in: |
Zur Gesamtaufnahme - volume:3 |
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Enthalten in: |
Micromachines - 3(2012), 3, Seite 574-581 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Philippe Renaud [VerfasserIn] |
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Links: |
doi.org [kostenfrei] |
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Themen: |
Carbon electrodes |
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doi: |
10.3390/mi3030574 |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
DOAJ060954248 |
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