Differences in Gene Expression Profile of Primary Tumors in Metastatic and Non-Metastatic Papillary Thyroid Carcinoma—Do They Exist?

Molecular mechanisms of distant metastases (M1) in papillary thyroid cancer (PTC) are poorly understood. We attempted to analyze the gene expression profile in PTC primary tumors to seek the genes associated with M1 status and characterize their molecular function. One hundred and twenty-three patients, including 36 M1 cases, were subjected to transcriptome oligonucleotide microarray analyses: (set A—U133, set B—HG 1.0 ST) at transcript and gene group level (limma, gene set enrichment analysis (GSEA)). An additional independent set of 63 PTCs, including 9 M1 cases, was used to validate results by qPCR. The analysis on dataset A detected eleven transcripts showing significant differences in expression between metastatic and non-metastatic PTC. These genes were validated on microarray dataset B. The differential expression was positively confirmed for only two genes: <i<IGFBP3, </i<(most significant) and <i<ECM1</i<. However, when analyzed on an independent dataset by qPCR, the <i<IGFBP3</i< gene showed no differences in expression. Gene group analysis showed differences mainly among immune-related transcripts, indicating the potential influence of tumor immune infiltration or signal within the primary tumor. The differences in gene expression profile between metastatic and non-metastatic PTC, if they exist, are subtle and potentially detectable only in large datasets..

Medienart:

E-Artikel

Erscheinungsjahr:

2020

Erschienen:

2020

Enthalten in:

Zur Gesamtaufnahme - volume:21

Enthalten in:

International Journal of Molecular Sciences - 21(2020), 4629, p 4629

Sprache:

Englisch

Beteiligte Personen:

Sylwia Szpak-Ulczok [VerfasserIn]
Aleksandra Pfeifer [VerfasserIn]
Dagmara Rusinek [VerfasserIn]
Malgorzata Oczko-Wojciechowska [VerfasserIn]
Malgorzata Kowalska [VerfasserIn]
Tomasz Tyszkiewicz [VerfasserIn]
Marta Cieslicka [VerfasserIn]
Daria Handkiewicz-Junak [VerfasserIn]
Krzysztof Fujarewicz [VerfasserIn]
Dariusz Lange [VerfasserIn]
Ewa Chmielik [VerfasserIn]
Ewa Zembala-Nozynska [VerfasserIn]
Sebastian Student [VerfasserIn]
Agnieszka Kotecka-Blicharz [VerfasserIn]
Aneta Kluczewska-Galka [VerfasserIn]
Barbara Jarzab [VerfasserIn]
Agnieszka Czarniecka [VerfasserIn]
Michal Jarzab [VerfasserIn]
Jolanta Krajewska [VerfasserIn]

Links:

doi.org [kostenfrei]
doaj.org [kostenfrei]
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Journal toc [kostenfrei]
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Themen:

Biology (General)
Chemistry
Distant metastases
Gene expression profile
Microarray
Papillary thyroid cancer

doi:

10.3390/ijms21134629

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

DOAJ060367032