Parasitic nematode-induced CD4+Foxp3+T cells can ameliorate allergic airway inflammation.
The recruitment of CD4+CD25+Foxp3+T (Treg) cells is one of the most important mechanisms by which parasites down-regulate the immune system.We compared the effects of Treg cells from Trichinella spiralis-infected mice and uninfected mice on experimental allergic airway inflammation in order to understand the functions of parasite-induced Treg cells. After four weeks of T. spiralis infection, we isolated Foxp3-GFP-expressing cells from transgenic mice using a cell sorter. We injected CD4+Foxp3+ cells from T. spiralis-infected [Inf(+)Foxp3+] or uninfected [Inf(-)Foxp3+] mice into the tail veins of C57BL/6 mice before the induction of inflammation or during inflammation. Inflammation was induced by ovalbumin (OVA)-alum sensitization and OVA challenge. The concentrations of the Th2-related cytokines IL-4, IL-5, and IL-13 in the bronchial alveolar lavage fluid and the levels of OVA-specific IgE and IgG1 in the serum were lower in mice that received intravenous application of Inf(+)Foxp3+ cells [IV(inf):+(+) group] than in control mice. Some features of allergic airway inflammation were ameliorated by the intravenous application of Inf(-)Foxp3+ cells [IV(inf):+(-) group], but the effects were less distinct than those observed in the IV(inf):+(+) group. We found that Inf(+)Foxp3+ cells migrated to inflammation sites in the lung and expressed higher levels of Treg-cell homing receptors (CCR5 and CCR9) and activation markers (Klrg1, Capg, GARP, Gzmb, OX40) than did Inf(-)Foxp3+ cells.T. spiralis infection promotes the proliferation and functional activation of Treg cells. Parasite-induced Treg cells migrate to the inflammation site and suppress immune responses more effectively than non-parasite-induced Treg cells. The adoptive transfer of Inf(+)Foxp3+ cells is an effective method for the treatment and prevention of allergic airway diseases in mice and is a promising therapeutic approach for the treatment of allergic airway diseases..
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2014 |
|---|---|
| Erschienen: |
2014 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:8 |
|---|---|
| Enthalten in: |
PLoS Neglected Tropical Diseases - 8(2014), 12, p e3410 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Shin Ae Kang [VerfasserIn] |
|---|
| Links: |
doi.org [kostenfrei] |
|---|
| Themen: |
Arctic medicine. Tropical medicine |
|---|
| doi: |
10.1371/journal.pntd.0003410 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
DOAJ044686145 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | DOAJ044686145 | ||
| 003 | DE-627 | ||
| 005 | 20230502090049.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 230227s2014 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1371/journal.pntd.0003410 |2 doi | |
| 035 | |a (DE-627)DOAJ044686145 | ||
| 035 | |a (DE-599)DOAJdd2999f51d3540e698e48c36287d0cfa | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 050 | 0 | |a RC955-962 | |
| 050 | 0 | |a RA1-1270 | |
| 100 | 0 | |a Shin Ae Kang |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Parasitic nematode-induced CD4+Foxp3+T cells can ameliorate allergic airway inflammation. |
| 264 | 1 | |c 2014 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 520 | |a The recruitment of CD4+CD25+Foxp3+T (Treg) cells is one of the most important mechanisms by which parasites down-regulate the immune system.We compared the effects of Treg cells from Trichinella spiralis-infected mice and uninfected mice on experimental allergic airway inflammation in order to understand the functions of parasite-induced Treg cells. After four weeks of T. spiralis infection, we isolated Foxp3-GFP-expressing cells from transgenic mice using a cell sorter. We injected CD4+Foxp3+ cells from T. spiralis-infected [Inf(+)Foxp3+] or uninfected [Inf(-)Foxp3+] mice into the tail veins of C57BL/6 mice before the induction of inflammation or during inflammation. Inflammation was induced by ovalbumin (OVA)-alum sensitization and OVA challenge. The concentrations of the Th2-related cytokines IL-4, IL-5, and IL-13 in the bronchial alveolar lavage fluid and the levels of OVA-specific IgE and IgG1 in the serum were lower in mice that received intravenous application of Inf(+)Foxp3+ cells [IV(inf):+(+) group] than in control mice. Some features of allergic airway inflammation were ameliorated by the intravenous application of Inf(-)Foxp3+ cells [IV(inf):+(-) group], but the effects were less distinct than those observed in the IV(inf):+(+) group. We found that Inf(+)Foxp3+ cells migrated to inflammation sites in the lung and expressed higher levels of Treg-cell homing receptors (CCR5 and CCR9) and activation markers (Klrg1, Capg, GARP, Gzmb, OX40) than did Inf(-)Foxp3+ cells.T. spiralis infection promotes the proliferation and functional activation of Treg cells. Parasite-induced Treg cells migrate to the inflammation site and suppress immune responses more effectively than non-parasite-induced Treg cells. The adoptive transfer of Inf(+)Foxp3+ cells is an effective method for the treatment and prevention of allergic airway diseases in mice and is a promising therapeutic approach for the treatment of allergic airway diseases. | ||
| 653 | 0 | |a Arctic medicine. Tropical medicine | |
| 653 | 0 | |a Public aspects of medicine | |
| 700 | 0 | |a Mi-Kyung Park |e verfasserin |4 aut | |
| 700 | 0 | |a Min Kyoung Cho |e verfasserin |4 aut | |
| 700 | 0 | |a Sang Kyun Park |e verfasserin |4 aut | |
| 700 | 0 | |a Min Seong Jang |e verfasserin |4 aut | |
| 700 | 0 | |a Bo-Gie Yang |e verfasserin |4 aut | |
| 700 | 0 | |a Myoung Ho Jang |e verfasserin |4 aut | |
| 700 | 0 | |a Dong-Hee Kim |e verfasserin |4 aut | |
| 700 | 0 | |a Hak Sun Yu |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i In |t PLoS Neglected Tropical Diseases |d Public Library of Science (PLoS), 2008 |g 8(2014), 12, p e3410 |w (DE-627)DOAJ000065781 |x 19352735 |7 nnns |
| 773 | 1 | 8 | |g volume:8 |g year:2014 |g number:12, p e3410 |
| 856 | 4 | 0 | |u https://doi.org/10.1371/journal.pntd.0003410 |z kostenfrei |
| 856 | 4 | 0 | |u https://doaj.org/article/dd2999f51d3540e698e48c36287d0cfa |z kostenfrei |
| 856 | 4 | 0 | |u http://europepmc.org/articles/PMC4270642?pdf=render |z kostenfrei |
| 856 | 4 | 2 | |u https://doaj.org/toc/1935-2727 |y Journal toc |z kostenfrei |
| 856 | 4 | 2 | |u https://doaj.org/toc/1935-2735 |y Journal toc |z kostenfrei |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_DOAJ | ||
| 912 | |a SSG-OLC-PHA | ||
| 951 | |a AR | ||
| 952 | |d 8 |j 2014 |e 12, p e3410 | ||